GABA-A R beta 2 Overexpression Lysate

Images

 
Western Blot: GABA A Receptor beta 2 Overexpression Lysate (Adult Normal) [NBL1-10921] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GABA A Receptor beta 2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GABA-A R beta 2 Overexpression Lysate Summary

Description

GABA-A R beta 2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC216424

Accession#: NM_000813

Protein Tag: C-MYC/DDK
Gene
GABRB2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
54.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GABA-A R beta 2 Overexpression Lysate

  • GABA A R beta 2
  • GABA(A) receptor subunit beta-2
  • GABAAR beta 2
  • GABAARb2
  • GABRB2
  • gamma-aminobutyric acid (GABA) A receptor, beta 2
  • gamma-aminobutyric acid A receptor beta 2
  • gamma-aminobutyric acid receptor subunit beta-2
  • MGC119386
  • MGC119388
  • MGC119389

Background

The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, beta 2 subunit. It is mapped to chromosome 5q34 in a cluster comprised of genes encoding alpha 1 and gamma 2 subunits of the GABA A receptor. Alternative splicing of this gene generates 2 transcript variants, differing by a 114 bp insertion. [provided by RefSeq]. Transcript Variant: This variant (2) lacks 114 bp within the coding region, as compared to variant 1, resulting in an isoform (2) which is 38 aa shorter. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-191
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IP, KO, WB
NB300-190
Species: Hu, Mu, Rt
Applications: IHC, KD, WB
NB300-196
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
NB300-199
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
NBP1-33284
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-59325
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
NBP2-38449
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-48319
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, MiAr, WB
640-A3
Species: Mu
Applications: Bind
NBP1-48272
Species: Hu, Pm, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, IP, PEP-ELISA, WB
NBP2-75497
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-88927
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB110-41404
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
NBP1-88191
Species: Hu, Mu
Applications: IHC, IHC-P, WB
H00728695-B01P
Species: Hu
Applications: ICC/IF, IP, WB
AF1188
Species: Rt
Applications: IHC, Simple Western, WB
NBP1-43435
Species: Hu, Mu
Applications: Flow, WB
H00006532-D01P
Species: Hu, Mu
Applications: WB

Publications for GABA-A R beta 2 Lysate (NBL1-10921) (0)

There are no publications for GABA-A R beta 2 Lysate (NBL1-10921).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GABA-A R beta 2 Lysate (NBL1-10921) (0)

There are no reviews for GABA-A R beta 2 Lysate (NBL1-10921). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GABA-A R beta 2 Lysate (NBL1-10921) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GABA-A R beta 2 Products

Bioinformatics Tool for GABA-A R beta 2 Lysate (NBL1-10921)

Discover related pathways, diseases and genes to GABA-A R beta 2 Lysate (NBL1-10921). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GABA-A R beta 2 Lysate (NBL1-10921)

Discover more about diseases related to GABA-A R beta 2 Lysate (NBL1-10921).
 

Pathways for GABA-A R beta 2 Lysate (NBL1-10921)

View related products by pathway.

PTMs for GABA-A R beta 2 Lysate (NBL1-10921)

Learn more about PTMs related to GABA-A R beta 2 Lysate (NBL1-10921).
 

Research Areas for GABA-A R beta 2 Lysate (NBL1-10921)

Find related products by research area.

Blogs on GABA-A R beta 2

There are no specific blogs for GABA-A R beta 2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GABA-A R beta 2 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol GABRB2