G6PC Overexpression Lysate

Images

 
Western Blot: G6PC Overexpression Lysate (Adult Normal) [NBP2-09053] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for G6PC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

G6PC Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_000142. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens G6PC, mRNA.
Gene
G6PC

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
40.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-09053 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for G6PC Overexpression Lysate

  • EC 3.1.3.9
  • G6Pase
  • G-6-Pase
  • G6Pase-alpha
  • G6PT
  • Glucose-6-phosphatase alpha
  • glucose-6-phosphatase
  • glucose-6-phosphatase, catalytic (glycogen storage disease type I, von Gierkedisease)
  • glucose-6-phosphatase, catalytic subunit
  • GSD1
  • GSD1a
  • MGC163350

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Applications: WB

Publications for G6PC Lysate (NBP2-09053) (0)

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Review for G6PC Lysate (NBP2-09053) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-09053:
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WB
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Images Ratings Applications Species Date Details
Western Blot G6PC NBP2-09053
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reviewed by:
Mohammad Abedi
WB Human 10/15/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman Neuroblastoma Cell Lines
SpeciesHuman
Lot09053

Blocking

Blocking Details5% milk solution overnight at 4oC

Primary Anitbody

Dilution Ratio1:1000, 1 hour Incubation at RT in TBST

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG- HRP Conjugated
Secondary Manufacturer Cat##7074S
Secondary Concentration1:1000 in TBST for 1 hour at RT

Details

Detection NotesDensitometry, VersaDoc MP 5000, 60 seconds

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for G6PC Lysate (NBP2-09053). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if glucose 6 phosphatase has any phosphatase activity? I am interested in the dephosphorylation of mannose phosphate.
    • NBP2-09053 is an overexpression lysate of G6PC overexpressed in 293T cells. It is unpurified and has never been tested for activity. It is mainly intended to be used as a positive control.

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Blogs on G6PC.

Glucose-6-phosphatase (G6PC) - A key to regulate your blood sugar level!
The integral endoplasmic reticulum membrane-based enzyme G6PC hydrolyzes its substrate glucose-6-phosphate into glucose. Specifically, G6PC breaks down D-glucose 6-phosphate to D-glucose and orthophosphate. Because G6PC forms with the glucose-6-phosph...  Read full blog post.

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Recent Reviews

5
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1
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1
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Mohammad Abedi
10/15/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol G6PC