G3BP1 Antibody

Images

 
Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Affinity purified rabbit anti-G3BP1 ...read more
Proximity Ligation Assay: G3BP1 Antibody [NBP1-18922] - Secondary -Conjugate duolink II PLA in Hela cells. goat anti-human RENT1 (NB100-370) and rabbit anti-human G3BP1 (NBP1-18922) image merged from DAPI (2 ms) and ...read more

Product Details

Summary
Reactivity Hu, Mu, MaSpecies Glossary
Applications WB, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

G3BP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human GTPase activating protein (SH3 domain) binding protein 1 using the numbering given in entry NP_005745.1 (GeneID 10146).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
G3BP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:200-1:2000
Publications
Read Publications using NBP1-18922.

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 28794026).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for G3BP1 Antibody

  • ATP-dependent DNA helicase VIII
  • EC 3.6.1
  • EC 3.6.4.12
  • EC 3.6.4.13
  • G3BP-1
  • G3BPRas-GTPase-activating protein SH3-domain-binding protein
  • GAP binding protein
  • GAP SH3 domain-binding protein 1
  • GTPase activating protein (SH3 domain) binding protein 1
  • hDH VIII
  • HDH-VIII
  • MGC111040
  • ras GTPase-activating protein-binding protein 1
  • RasGAP-associated endoribonuclease G3BP

Background

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for G3BP1 Antibody (NBP1-18922)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol G3BP1
Entrez
Uniprot