G3BP1 Antibody

Images

 
Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate (50 ug) from HeLa, MCF-7, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP1antibody used for ...read more
Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody [NBP1-18922] - G3BP1 in red (Alexa fluor 555) and nucleus in blue (DAPI). Human primary fibroblasts untreated in the left and treated in the right with G3BP1 ...read more
Independent Antibodies: Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. ...read more

Product Details

Summary
Reactivity Hu, Mu, Ma, Pm-OrSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

G3BP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human GTPase activating protein (SH3 domain) binding protein 1 using the numbering given in entry NP_005745.1 (GeneID 10146).
Predicted Species
Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
G3BP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunoprecipitation 2-10 ug/mg lysate
Application Notes
G3BP1 antibody validated for ICC/IF from a verified customer review.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-18922 in the following applications:

Publications
Read Publications using
NBP1-18922 in the following applications:

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 28794026).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for G3BP1 Antibody

  • ATP-dependent DNA helicase VIII
  • EC 3.6.1
  • EC 3.6.4.12
  • EC 3.6.4.13
  • G3BP-1
  • G3BPRas-GTPase-activating protein SH3-domain-binding protein
  • GAP binding protein
  • GAP SH3 domain-binding protein 1
  • GTPase activating protein (SH3 domain) binding protein 1
  • hDH VIII
  • HDH-VIII
  • MGC111040
  • ras GTPase-activating protein-binding protein 1
  • RasGAP-associated endoribonuclease G3BP

Background

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-67203
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF5094
Species: Hu, Mu, Rt
Applications: WB
NBP1-88195
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP1-82977
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB600-335
Species: Hu, Mu, Ec, All-NA
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-13509
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01452
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, KO
NB120-6125
Species: Hu, Bv, Ca, Pm, Xp, Dr(-), Mu(-)
Applications: WB, ELISA, Flow, ICC/IF, IP, MiAr, CyTOF-ready
AF2226
Species: Hu
Applications: WB, Simple Western, ICC
NB100-82245
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P, KD
NBP1-82520
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-71743
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
NBP1-79932
Species: Hu, Mu, Ze
Applications: WB, ICC/IF, ISH
H00023435-M01
Species: Hu, Mu, Rt, Ba, Pp, I, Pm
Applications: WB, ELISA, Func, ICC/IF, IHC, IHC-P, IP, RNAi, S-ELISA
NBP1-56484
Species: Hu
Applications: WB, IHC, IHC-P
NB100-268
Species: Hu, Po
Applications: WB, IP
AF1457
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, KO

Publications for G3BP1 Antibody (NBP1-18922)(3)

We have publications tested in 2 confirmed species: Human, Mammal.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
All Applications
Filter By Species
Human
(1)
Mammal
(1)
All Species

Review for G3BP1 Antibody (NBP1-18922) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-18922:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry G3BP1 NBP1-18922
Enlarge
5
reviewed by:
Anonymous
ICC Human 02/12/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman primary fibroblast
SpeciesHuman
LotA1

Comments

CommentsICC-IF
Fixation: PFA 4% 10min RT
permeabilization: Triton 0.1% TBS
blocking: Donkey Serum 10% TBS-T
1. Ab dilution: 1:1000
Secondary Ab: Alexa fluor 555 Donkey anti-rabbit 1:600
Mounting medium with DAPI

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for G3BP1 Antibody (NBP1-18922) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional G3BP1 Products

Bioinformatics Tool for G3BP1 Antibody (NBP1-18922)

Discover related pathways, diseases and genes to G3BP1 Antibody (NBP1-18922). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for G3BP1 Antibody (NBP1-18922)

Discover more about diseases related to G3BP1 Antibody (NBP1-18922).
 

Pathways for G3BP1 Antibody (NBP1-18922)

View related products by pathway.

PTMs for G3BP1 Antibody (NBP1-18922)

Learn more about PTMs related to G3BP1 Antibody (NBP1-18922).
 

Research Areas for G3BP1 Antibody (NBP1-18922)

Find related products by research area.

Blogs on G3BP1

There are no specific blogs for G3BP1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
02/12/2019
Application: ICC
Species: Human

Bioinformatics

Gene Symbol G3BP1
Entrez
Uniprot