USP10 Antibody (OTI2E1)

Images

 
Genetic Strategies: Western Blot: USP10 Antibody (OTI2E1) [NBP2-01452] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and USP10-Knockout Hela cells (KO) were separated by ...read more
Immunocytochemistry/ Immunofluorescence: USP10 Antibody (2E1) [NBP2-01452] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY USP10.
Immunohistochemistry-Paraffin: USP10 Antibody (OTI2E1) [NBP2-01452] - Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced ...read more
Flow Cytometry: USP10 Antibody (2E1) [NBP2-01452] - Analysis of Hela cells, using anti-USP10 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: USP10 Antibody (2E1) [NBP2-01452] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Western Blot: USP10 Antibody (2E1) [NBP2-01452] Analysis of extracts (35ug) from 9 different cell lines by using anti-USP10 monoclonal antibody.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-USP10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-USP10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human breast tissue using anti-USP10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human endometrium tissue using anti-USP10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human lymphoma tissue using anti-USP10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human pancreas tissue using anti-USP10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human prostate tissue using anti-USP10 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, KO
Clone
OTI2E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

USP10 Antibody (OTI2E1) Summary

Immunogen
Full length human recombinant protein of human USP10(NP_005144) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
USP10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Knockout Validated
  • Western Blot 1:500-2000
Theoretical MW
87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-01452 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for USP10 Antibody (OTI2E1)

  • Deubiquitinating enzyme 10
  • EC 3.4.19.12
  • KIAA0190MGC2621
  • ubiquitin carboxyl-terminal hydrolase 10
  • ubiquitin specific peptidase 10
  • ubiquitin specific protease 10
  • ubiquitin thioesterase 10
  • Ubiquitin Thiolesterase 10
  • Ubiquitin-specific-processing protease 10
  • UBPO
  • Uchrp
  • USP10

Background

Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP10 Antibody (NBP2-01452)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol USP10
Entrez