USP10 Antibody (OTI2E1)

Western Blot: USP10 Antibody (OTI2E1) [NBP2-01452] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and USP10-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-USP10 monoclonal antibody NBP2-01452, (1:500). Then the blotted membrane was stripped and reprobed with an anti-beta-actin antibody as a loading control.

Immunocytochemistry/Immunofluorescence: USP10 Antibody (2E1) [NBP2-01452] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY USP10.

Immunohistochemistry-Paraffin: USP10 Antibody (OTI2E1) [NBP2-01452] - Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP2-01452)

Flow Cytometry: USP10 Antibody (2E1) [NBP2-01452] - Analysis of Hela cells, using anti-USP10 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: USP10 Antibody (2E1) [NBP2-01452] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP10.

Western Blot: USP10 Antibody (2E1) [NBP2-01452] Analysis of extracts (35ug) from 9 different cell lines by using anti-USP10 monoclonal antibody.

Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-USP10 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-USP10 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human breast tissue using anti-USP10 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human endometrium tissue using anti-USP10 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human lymphoma tissue using anti-USP10 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human pancreas tissue using anti-USP10 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human prostate tissue using anti-USP10 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, KO
• Clone: OTI2E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

USP10 Antibody (OTI2E1) Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse USP10 Antibody (OTI2E1) (NBP2-01452) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-USP10 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human USP10(NP_005144) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Knockout Validated
  • Western Blot 1:500-2000
• Theoretical MW: 87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP10 Antibody (OTI2E1)

• Deubiquitinating enzyme 10
• EC 3.4.19.12
• KIAA0190MGC2621
• ubiquitin carboxyl-terminal hydrolase 10
• ubiquitin specific peptidase 10
• ubiquitin specific protease 10
• ubiquitin thioesterase 10
• Ubiquitin Thiolesterase 10
• Ubiquitin-specific-processing protease 10
• UBPO
• Uchrp
• USP10

Background

Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP10 Antibody (NBP2-01452)(1)

Publication using NBP2-01452

• Xia X, Liu X, Chai R et al. USP10 exacerbates neointima formation by stabilizing Skp2 protein in vascular smooth muscle cells Journal of Biological Chemistry 2021-09-01 [PMID: 34599966] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: USP10
• Entrez:
  • 9100(Human)