ARL2BP Antibody (OTI1A10)

Images

 
Western Blot: ARL2BP Antibody (OTI1A10) [NBP2-46541] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL2BP .
Immunohistochemistry: ARL2BP Antibody (OTI1A10) [NBP2-46541] - Analysis of Human Kidney tissue.
Flow Cytometry: ARL2BP Antibody (OTI1A10) [NBP2-46541] - Analysis of HEK293T cells transfected with either RC200198 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by ARL2BP antibody, ...read more
Immunohistochemistry: ARL2BP Antibody (OTI1A10) [NBP2-46541] - Analysis of Carcinoma of Human prostate tissue.
Immunohistochemistry: ARL2BP Antibody (OTI1A10) [NBP2-46541] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: ARL2BP Antibody (OTI1A10) [NBP2-46541] - Analysis of Adenocarcinoma of Human colon tissue.
Immunohistochemistry: ARL2BP Antibody (OTI1A10) [NBP2-46541] - Analysis of Human pancreas tissue.
Immunohistochemistry: ARL2BP Antibody (OTI1A10) [NBP2-46541] - Analysis of Carcinoma of Human thyroid tissue.
Immunofluorescence: ARL2BP Antibody (OTI1A10) [NBP2-46541] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ARL2BP.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, ICC/IF
Clone
OTI1A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ARL2BP Antibody (OTI1A10) Summary

Immunogen
Full length human recombinant protein of human ARL2BP (NP_036238) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ARL2BP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
18.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARL2BP Antibody (OTI1A10)

  • ADP-ribosylation factor-like 2 binding protein
  • ADP-ribosylation factor-like protein 2-binding protein
  • ARF-like 2-binding protein
  • BART1binder of Arl Two
  • BARTArf-like 2 binding protein BART1
  • Binder of ARF2 protein 1
  • binder of Arl2

Background

ADP-ribosylation factor (ARF)-like proteins (ARLs) comprise a functionally distinct group of the ARF family of RAS-related GTPases. The protein encoded by this gene binds to ARL2.GTP with high affinity but does not interact with ARL2.GDP, activated ARF, or RHO proteins. The lack of detectable membrane association of this protein or ARL2 upon activation of ARL2 is suggestive of actions distinct from those of the ARFs. This protein is considered to be the first ARL2-specific effector identified, due to its interaction with ARL2.GTP but lack of ARL2 GTPase-activating protein activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ARL2BP