G3BP1 Antibody (HL2254) - Azide and BSA Free

Immunohistochemistry-Paraffin: G3BP1 Antibody (HL2254) - Azide and BSA Free [NBP3-25472] - G3BP1 antibody [HL2254] detects G3BP1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. G3BP1 stained by G3BP1 antibody [HL2254] (NBP3-25472) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: G3BP1 Antibody (HL2254) - Azide and BSA Free [NBP3-25472] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with G3BP1 antibody [HL2254] (NBP3-25472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: G3BP1 Antibody (HL2254) - Azide and BSA Free [NBP3-25472] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with G3BP1 antibody [HL2254] (NBP3-25472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: G3BP1 Antibody (HL2254) - Azide and BSA Free [NBP3-25472] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with G3BP1 antibody [HL2254] (NBP3-25472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: G3BP1 Antibody (HL2254) - Azide and BSA Free [NBP3-25472] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with G3BP1 antibody [HL2254] (NBP3-25472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: G3BP1 Antibody (HL2254) - Azide and BSA Free [NBP3-25472] - G3BP1 antibody [HL2254] detects G3BP1 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: G3BP1 stained by G3BP1 antibody [HL2254] (NBP3-25472) diluted at 1:500. Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: HL2254
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

G3BP1 Antibody (HL2254) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit G3BP1 Antibody (HL2254) - Azide and BSA Free (NBP3-25472) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human G3BP1.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: G3BP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for G3BP1 Antibody (HL2254) - Azide and BSA Free

• ATP-dependent DNA helicase VIII
• EC 3.6.1
• EC 3.6.4.12
• EC 3.6.4.13
• G3BP-1
• G3BPRas-GTPase-activating protein SH3-domain-binding protein
• GAP binding protein
• GAP SH3 domain-binding protein 1
• GTPase activating protein (SH3 domain) binding protein 1
• hDH VIII
• HDH-VIII
• MGC111040
• ras GTPase-activating protein-binding protein 1
• RasGAP-associated endoribonuclease G3BP

Background

G3BP1 encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: G3BP1