G protein alpha inhibitor 1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: G protein alpha inhibitor 1 Antibody [NBP2-59000] - Staining of human cell line A-431 shows localization to centrosome.

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-59000] -Staining of human cerebral cortex shows moderate membranous-cytoplasmic positivity in neuropil and astrocytes.

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-59000] -Staining of human kidney shows strong membranous-cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-59000] -Staining of human pancreas shows moderate membranous/cytoplasmic positivity in endocrine glandular cells.

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-59000] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

G protein alpha inhibitor 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit G protein alpha inhibitor 1 Antibody - BSA Free (NBP2-59000) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ARQLFVLAGAAEEGFMTAELAGVIKR
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNAI1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. IHC, Retrieval method: HIER pH6.
• Control Peptide:

  G protein alpha inhibitor 1 Recombinant Protein Antigen (NBP2-59000PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for G protein alpha inhibitor 1 Antibody - BSA Free

• Adenylate cyclase-inhibiting G alpha protein
• Gi
• Gi1 protein alpha subunit
• guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 1
• guanine nucleotide-binding protein G(i) subunit alpha-1

Background

Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNAI1