G protein alpha Antibody (OTI12D9) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB
• Clone: OTI12D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

G protein alpha Antibody (OTI12D9) [Alexa Fluor® 488] Summary

• Immunogen: Full length recombinant protein of human G protein alpha (NP_000507) produced in HEK293T cells.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GNAS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for G protein alpha Antibody (OTI12D9) [Alexa Fluor® 488]

• Adenylate cyclase-stimulating G alpha protein
• AHO
• Alternative gene product encoded by XL-exon
• Extra large alphas protein
• GNAS complex locus
• GNAS1GSA
• GNASXL
• GPSAC20orf45
• GSP
• guanine nucleotide binding protein (G protein), alpha stimulating activitypolypeptide 1
• guanine nucleotide regulatory protein
• guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas
• MGC33735
• NESP
• NESP55
• neuroendocrine secretory protein
• PHP1A
• PHP1B
• PHP1C
• POH
• protein ALEX
• SCG6
• secretogranin VI
• XLalphas

Background

G protein alpha has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNAS