G protein alpha Antibody (2A2B7) - BSA Free

Western Blot: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis using GNAS mAb against human GNAS (AA: 42-188) recombinant protein. (Expected MW is 42.8 kDa)

Immunohistochemistry: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis of paraffin-embedded breast cancer tissues using GNAS mouse mAb with DAB staining.

Flow Cytometry: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis of A549 cells using GNAS mouse mAb (green) and negative control (red).

Western Blot: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis using GNAS mAb against HEK293 (1) and GNAS (AA: 42-188)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis of paraffin-embedded lung cancer tissues using GNAS mouse mAb with DAB staining.

ELISA: G protein alpha Antibody (2A2B7) [NBP2-52477] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready
• Clone: 2A2B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

G protein alpha Antibody (2A2B7) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human GNAS (AA: 42-188) expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GNAS
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for G protein alpha Antibody (2A2B7) - BSA Free

• Adenylate cyclase-stimulating G alpha protein
• AHO
• Alternative gene product encoded by XL-exon
• Extra large alphas protein
• GNAS complex locus
• GNAS1GSA
• GNASXL
• GPSAC20orf45
• GSP
• guanine nucleotide binding protein (G protein), alpha stimulating activitypolypeptide 1
• guanine nucleotide regulatory protein
• guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas
• MGC33735
• NESP
• NESP55
• neuroendocrine secretory protein
• PHP1A
• PHP1B
• PHP1C
• POH
• protein ALEX
• SCG6
• secretogranin VI
• XLalphas

Background

G protein alpha has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for G protein alpha Antibody (NBP2-52477)(1)

Publication using NBP2-52477

• Mystek P, Rysiewicz B, Gregrowicz J et al. G gamma and G alpha Identity Dictate a G-Protein Heterotrimer Plasma Membrane Targeting Cells 2019-10-13 [PMID: 31614907] (WB, Human)

Bioinformatics

• Gene Symbol: GNAS