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G protein alpha Antibody (2A2B7) - BSA Free

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Western Blot: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis using GNAS mAb against human GNAS (AA: 42-188) recombinant protein. (Expected MW is 42.8 kDa)
Western Blot: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis using GNAS mAb against human GNAS (AA: 42-188) recombinant protein. (Expected MW is 42.8 kDa)
Immunohistochemistry: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis of paraffin-embedded breast cancer tissues using GNAS mouse mAb with DAB staining.
Flow Cytometry: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis of A549 cells using GNAS mouse mAb (green) and negative control (red).
Western Blot: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis using GNAS mAb against HEK293 (1) and GNAS (AA: 42-188)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis of paraffin-embedded lung cancer tissues using GNAS mouse mAb with DAB staining.
ELISA: G protein alpha Antibody (2A2B7) [NBP2-52477] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
Clone
2A2B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52477-0.025mg
100% Guarantee on every product

G protein alpha Antibody (2A2B7) - BSA Free Summary

Immunogen
Purified recombinant fragment of human GNAS (AA: 42-188) expressed in E. Coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GNAS
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-52477 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for G protein alpha Antibody (2A2B7) - BSA Free

  • Adenylate cyclase-stimulating G alpha protein
  • AHO
  • Alternative gene product encoded by XL-exon
  • Extra large alphas protein
  • GNAS complex locus
  • GNAS1GSA
  • GNASXL
  • GPSAC20orf45
  • GSP
  • guanine nucleotide binding protein (G protein), alpha stimulating activitypolypeptide 1
  • guanine nucleotide regulatory protein
  • guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas
  • MGC33735
  • NESP
  • NESP55
  • neuroendocrine secretory protein
  • PHP1A
  • PHP1B
  • PHP1C
  • POH
  • protein ALEX
  • SCG6
  • secretogranin VI
  • XLalphas

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.
Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction. Nuclei in IF experiments (A-D) were stained with DAPI (cyan), and with nuclear fast red in E and F. At low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer through the inner nuclear layer. No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).
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Simple Western: Syntaxin 16 Antibody [NBP1-92467] - Electropherogram image of the corresponding Simple Western lane view. Syntaxin 16 antibody was used at 1:100 dilution on MCF-7 lysate(s) respectively.
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Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
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G protein alpha
Antibody (2A2B7) - BS ...
NBP2-52477
G protein alpha Antibody (2A2B7) - BSA Free
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready

Publications for G protein alpha Antibody (NBP2-52477)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol GNAS