G-CSFR/CD114 Antibody (38643)

Images

 
Western blot shows lysates of AML‑193 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human G‑CSF R/CD114 Monoclonal Antibody (Catalog # MAB3811) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ELISA(Cap)
Clone
38643
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

G-CSFR/CD114 Antibody (38643) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human G-CSF R/CD114
Glu25-Pro621
Accession # Q99062
Specificity
Detects human G-CSF R/CD114 in ELISAs and Western blots. In sandwich Immunoassays, no cross-reactivity with recombinant human (rh) G‑CSF, rhGM‑CSF R beta, rhGM‑CSF R, or recombinant mouse G‑CSF is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CSF3R
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human G-CSF R/CD114 Biotinylated Antibody (Catalog number BAF381)
Standard: Recombinant Human G-CSF R/CD114 (Catalog number 381-GR)
Publications
Read Publications using
MAB3811 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for G-CSFR/CD114 Antibody (38643)

  • CD114 antigen
  • CD114
  • colony stimulating factor 3 receptor (granulocyte)
  • CSF3R
  • Csfgr
  • G-CSF R
  • G-CSF receptor
  • GCSFR
  • G-CSFR
  • GCSFRG-CSF-R
  • granulocyte colony-stimulating factor receptor

Background

Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant. Cell activation by G-CSF is mediated by a type I membrane protein belonging to the cytokine receptor superfamily. Human G-CSF R is 863 amino acids (aa) in length, with a 604 aa extracellular domain, a 26 aa transmembrane domain, and a 183 aa cytoplasmic domain that include a 23 amino acid signal sequence. As a result of alternative splicing, at least four isoforms of G-CSF R that differ in their C-terminal region exist. Isoform 2 lacks the transmembrane region and may represent a soluble form of the receptor; however the existence of soluble G-CSF R in human serum has not been reported (1). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia. These mutations typically led to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophil precursors in the bone marrow and neutropenia in peripheral blood (2). Human and mouse G-CSF R have a homology of 62.5%. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta. Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases. Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3, STAT3, and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (1).

  1. Nicola, N.A., in Cytokine Reference, (2001) Oppenhiem, J.J. and M. Feldmann, eds. Academic Press p. 1935
  2. Mitsui, T. et al. (2003) Blood. 101:2990.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for G-CSFR/CD114 Antibody (MAB3811)(2)

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB3811U
Alexa Fluor 405 FAB3811V
Alexa Fluor 488 FAB3811G
Alexa Fluor 594 FAB3811T
Alexa Fluor 647 FAB3811R
Alexa Fluor 700 FAB3811N
Alexa Fluor 750 FAB3811S

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Bioinformatics

Gene Symbol CSF3R
Uniprot