G-CSFR/CD114 Antibody (38643)

Images

 
Western blot shows lysates of AML‑193 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human G‑CSF R/CD114 Monoclonal Antibody (Catalog # MAB3811) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ELISA(Cap)
Clone
38643
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

G-CSFR/CD114 Antibody (38643) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human G-CSF R/CD114
Glu25-Pro621
Accession # Q99062
Specificity
Detects human G-CSF R/CD114 in ELISAs and Western blots. In sandwich Immunoassays, no cross-reactivity with recombinant human (rh) G‑CSF, rhGM‑CSF R beta , rhGM‑CSF R, or recombinant mouse G‑CSF is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CSF3R
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.5 ug/mL
Application Notes
ELISA Detection: Human G-CSF R/CD114 Biotinylated Antibody (Catalog number BAF381)
Standard: Recombinant Human G-CSF R/CD114 (Catalog number 381-GR)
Publications
Read Publications using
MAB3811 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for G-CSFR/CD114 Antibody (38643)

  • CD114 antigen
  • CD114
  • colony stimulating factor 3 receptor (granulocyte)
  • CSF3R
  • Csfgr
  • G-CSF R
  • G-CSF receptor
  • GCSFR
  • G-CSFR
  • GCSFRG-CSF-R
  • granulocyte colony-stimulating factor receptor

Background

Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant. Cell activation by G-CSF is mediated by a type I membrane protein belonging to the cytokine receptor superfamily. Human G-CSF R is 863 amino acids (aa) in length, with a 604 aa extracellular domain, a 26 aa transmembrane domain, and a 183 aa cytoplasmic domain that include a 23 amino acid signal sequence. As a result of alternative splicing, at least four isoforms of G-CSF R that differ in their C-terminal region exist. Isoform 2 lacks the transmembrane region and may represent a soluble form of the receptor; however the existence of soluble G-CSF R in human serum has not been reported (1). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia. These mutations typically led to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophil precursors in the bone marrow and neutropenia in peripheral blood (2). Human and mouse G-CSF R have a homology of 62.5%. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta. Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases. Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3, STAT3, and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (1).

  1. Nicola, N.A., in Cytokine Reference, (2001) Oppenhiem, J.J. and M. Feldmann, eds. Academic Press p. 1935
  2. Mitsui, T. et al. (2003) Blood. 101:2990.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MCS00
Species: Mu
Applications: ELISA
203-IL/CF
Species: Hu
Applications: BA
MCS00
Species: Mu
Applications: ELISA
MAB1799
Species: Hu, Mu, Rt
Applications: ICC, IP, ICFlow, KO, WB
MAB9167
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
NBP2-59451
Species: Hu
Applications: ELISA, Flow, ICC/IF, MiAr, WB
AF1584
Species: Hu, Mu
Applications: CyTOF-ready, ICC, IP, ICFlow, KO, Simple Western, WB
AF2168
Species: Hu, Mu
Applications: ChIP, Simple Western, WB
D6050
Species: Hu
Applications: ELISA
NB600-1071
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
DGP00
Species: Hu
Applications: ELISA
DY3667
Species: Mu
Applications: ELISA
NB400-141
Species: Ha, Hu, Mu
Applications: ICC/IF, IP, WB
7954-GM/CF
Species: Hu
Applications: BA
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
7268-CT
Species: Hu
Applications: BA
NBP1-83349
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
7954-GM/CF
Species: Hu
Applications: BA
MAB3811
Species: Hu
Applications: WB, Flow, CyTOF-ready, ELISA(Cap)

Publications for G-CSFR/CD114 Antibody (MAB3811)(2)

Reviews for G-CSFR/CD114 Antibody (MAB3811) (0)

There are no reviews for G-CSFR/CD114 Antibody (MAB3811). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for G-CSFR/CD114 Antibody (MAB3811) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional G-CSFR/CD114 Products

Bioinformatics Tool for G-CSFR/CD114 Antibody (MAB3811)

Discover related pathways, diseases and genes to G-CSFR/CD114 Antibody (MAB3811). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for G-CSFR/CD114 Antibody (MAB3811)

Discover more about diseases related to G-CSFR/CD114 Antibody (MAB3811).
 

Pathways for G-CSFR/CD114 Antibody (MAB3811)

View related products by pathway.

PTMs for G-CSFR/CD114 Antibody (MAB3811)

Learn more about PTMs related to G-CSFR/CD114 Antibody (MAB3811).

Blogs on G-CSFR/CD114

There are no specific blogs for G-CSFR/CD114, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our G-CSFR/CD114 Antibody (38643) and receive a gift card or discount.

Bioinformatics

Gene Symbol CSF3R
Uniprot