G-CSF Overexpression Lysate

Images

 
Western Blot: G-CSF Overexpression Lysate (Adult Normal) [NBL1-09519] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for G-CSF.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

G-CSF Overexpression Lysate Summary

Description

G-CSF Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC207709

Accession#: NM_172220

Protein Tag: C-MYC/DDK
Gene
CSF3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for G-CSF Overexpression Lysate

  • C17orf33
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
  • CSF3
  • CSF3OS
  • Filgrastim
  • GCSF
  • G-CSF
  • GCSFlenograstim
  • granulocyte colony-stimulating factor
  • Lenograstim
  • MGC45931
  • Pluripoietin

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (3) uses an alternate in-frame splice site and contains an alternate segment which results in a frameshift, compared to variant 1. The resulting predicted isoform (c) is shorter, lacks a signal peptide, and has a distinct N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MCS00
Species: Mu
Applications: ELISA
7954-GM/CF
Species: Hu
Applications: BA
203-IL/CF
Species: Hu
Applications: BA
D6050
Species: Hu
Applications: ELISA
NB600-1071
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
7954-GM/CF
Species: Hu
Applications: BA
255-SC/CF
Species: Hu
Applications: BA
MEP00B
Species: Mu
Applications: ELISA
NBP2-42388
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
7268-CT
Species: Hu
Applications: BA
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
MAB3811
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, WB
202-IL
Species: Hu
Applications: BA
288-TPN/CF
Species: Hu
Applications: BA
6507-IL/CF
Species: Hu
Applications: BA
201-LB/CF
Species: Hu
Applications: BA
DY417
Species: Mu
Applications: ELISA
MAB208
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB

Publications for G-CSF Lysate (NBL1-09519) (0)

There are no publications for G-CSF Lysate (NBL1-09519).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for G-CSF Lysate (NBL1-09519) (0)

There are no reviews for G-CSF Lysate (NBL1-09519). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for G-CSF Lysate (NBL1-09519) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional G-CSF Products

Bioinformatics Tool for G-CSF Lysate (NBL1-09519)

Discover related pathways, diseases and genes to G-CSF Lysate (NBL1-09519). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for G-CSF Lysate (NBL1-09519)

Discover more about diseases related to G-CSF Lysate (NBL1-09519).
 

Pathways for G-CSF Lysate (NBL1-09519)

View related products by pathway.

PTMs for G-CSF Lysate (NBL1-09519)

Learn more about PTMs related to G-CSF Lysate (NBL1-09519).
 

Research Areas for G-CSF Lysate (NBL1-09519)

Find related products by research area.

Blogs on G-CSF

There are no specific blogs for G-CSF, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our G-CSF Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol CSF3