Human G-CSF ELISA Kit (Colorimetric) Summary
Sensitivity |
1 pg/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 12% |
Intra-Assay |
CV% < 10% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
CSF3 |
Applications/Dilutions
Dilutions |
|
Application Notes |
CSF3 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CSF3. |
Reactivity Notes
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Assay Diluent A (Item D): 0.09% sodium azide as preservative. ForStandard/Sample (serum/plasma) diluent.
- Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample(cell culture medium/urine) diluent.
- Detection Antibody GCSF (Item F): biotinylated anti-human GCSF (eachvial is enough to assay half microplate)
- G-CSF Microplate (Item A): coated with anti-human G-CSF
- HRP-Streptavidin Concentrate (Item G): 260x concentratedHRP-conjugated streptavidin.
- Standards (Item C): recombinant human G-CSF
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human G-CSF ELISA Kit (Colorimetric)
Background
Granulocyte Colony-Stimulating Factor (G-CSF filgrastim, pegfilgrastim or lenograstim) is a hematopoietic growth factor that stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage, in a dose-dependent manner. It is produced by a variety of cells including macrophages, monocytes, fibroblasts, endothelial cells and bone marrow stroma in response to specific stimulation by f.ex. endotoxin, TNF-alpha and IFN-gamma. Clinical applications of G-CSF include treatment of leukopenia following cancer therapy and bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant human G-CSF is a 18.7 kDa glycoprotein containing 174 amino acid residues.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for G-CSF ELISA Kit (KA1718). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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