Human G-CSF ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1718
    • Availability
      Product Discontinued

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Human G-CSF ELISA Kit (Colorimetric) Summary

Sensitivity
1 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CSF3

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
CSF3 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CSF3.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): 0.09% sodium azide as preservative. ForStandard/Sample (serum/plasma) diluent.
  2. Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample(cell culture medium/urine) diluent.
  3. Detection Antibody GCSF (Item F): biotinylated anti-human GCSF (eachvial is enough to assay half microplate)
  4. G-CSF Microplate (Item A): coated with anti-human G-CSF
  5. HRP-Streptavidin Concentrate (Item G): 260x concentratedHRP-conjugated streptavidin.
  6. Standards (Item C): recombinant human G-CSF
  7. Stop Solution (Item I): 0.2 M sulfuric acid.
  8. TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human G-CSF ELISA Kit (Colorimetric)

  • C17orf33
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
  • CSF3
  • CSF3OS
  • Filgrastim
  • GCSF
  • G-CSF
  • GCSFlenograstim
  • granulocyte colony-stimulating factor
  • Lenograstim
  • MGC45931
  • Pluripoietin

Background

Granulocyte Colony-Stimulating Factor (G-CSF filgrastim, pegfilgrastim or lenograstim) is a hematopoietic growth factor that stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage, in a dose-dependent manner. It is produced by a variety of cells including macrophages, monocytes, fibroblasts, endothelial cells and bone marrow stroma in response to specific stimulation by f.ex. endotoxin, TNF-alpha and IFN-gamma. Clinical applications of G-CSF include treatment of leukopenia following cancer therapy and bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant human G-CSF is a 18.7 kDa glycoprotein containing 174 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for G-CSF ELISA Kit (KA1718). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol CSF3