Human G-CSF ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1718
    • Availability
      Product Discontinued

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Human G-CSF ELISA Kit (Colorimetric) Summary

Sensitivity
1 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CSF3

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
CSF3 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CSF3.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): 0.09% sodium azide as preservative. ForStandard/Sample (serum/plasma) diluent.
  2. Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample(cell culture medium/urine) diluent.
  3. Detection Antibody GCSF (Item F): biotinylated anti-human GCSF (eachvial is enough to assay half microplate)
  4. G-CSF Microplate (Item A): coated with anti-human G-CSF
  5. HRP-Streptavidin Concentrate (Item G): 260x concentratedHRP-conjugated streptavidin.
  6. Standards (Item C): recombinant human G-CSF
  7. Stop Solution (Item I): 0.2 M sulfuric acid.
  8. TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human G-CSF ELISA Kit (Colorimetric)

  • C17orf33
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
  • CSF3
  • CSF3OS
  • Filgrastim
  • GCSF
  • G-CSF
  • GCSFlenograstim
  • granulocyte colony-stimulating factor
  • Lenograstim
  • MGC45931
  • Pluripoietin

Background

Granulocyte Colony-Stimulating Factor (G-CSF , filgrastim, pegfilgrastim or lenograstim) is a hematopoietic growth factor that stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage, in a dose-dependent manner. It is produced by a variety of cells including macrophages, monocytes, fibroblasts, endothelial cells and bone marrow stroma in response to specific stimulation by f.ex. endotoxin, TNF-alpha and IFN-gamma. Clinical applications of G-CSF include treatment of leukopenia following cancer therapy and bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant human G-CSF is a 18.7 kDa glycoprotein containing 174 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for G-CSF ELISA Kit (KA1718). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol CSF3