Fyn Antibody

Images

 
Western Blot: Fyn Antibody [NBP1-32067] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Fyn Antibody [NBP1-32067] - analysis of A431, using FYN antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Fyn Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human FYN. The exact sequence is proprietary.
Localization
Cell membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FYN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-32067 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (96%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 40% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fyn Antibody

  • EC 2.7.10
  • EC 2.7.10.2
  • FYN oncogene related to SRC, FGR, YES
  • Fyn
  • MGC45350
  • OKT3-induced calcium influx regulator
  • p59-Fyn
  • protein-tyrosine kinase fyn
  • Proto-oncogene c-Fyn
  • Proto-oncogene Syn
  • proto-oncogene tyrosine-protein kinase fyn
  • SLK
  • SLKc-syn protooncogene
  • src/yes-related novel
  • Src-like kinase
  • SYN
  • tyrosine kinase p59fyn(T)
  • tyrosine-protein kinase Fyn

Background

Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1. The unique N terminal domain of Fyn interacts with the CD3 and eta chains of the TcR. Fyn can bind to other proteins (p82 and p116) through its SH2 and SH3 domains, which may act as substrates or regulators of Fyn activity. Fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Fyn Antibody (NBP1-32067) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-32067:
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Images Ratings Applications Species Date Details
Western Blot Fyn NBP1-32067
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reviewed by:
Hongxia Hu
WB Human 09/12/2012
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Summary

ApplicationWestern Blot
Sample TestedWCL
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Recent Reviews

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5
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4
1
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0
2
0
1
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Hongxia Hu
09/12/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol FYN
Entrez