Yes Antibody (339827)

Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Yes Monoclonal Antibody (Catalog # MAB32051) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Yes at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Yes was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human/Mouse/Rat Yes Monoclonal Antibody (Catalog # MAB32051) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 339827
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Yes Antibody (339827) Summary

• Immunogen: E. coli-derived recombinant human Yes
  Met1-Gly88
  Accession # P07947.3
• Specificity: Detects endogenous human, mouse, and rat Yes in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: YES1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Yes Antibody (339827)

• cellular yes-1 protein
• c-yes
• EC 2.7.10
• EC 2.7.10.2
• HsT441
• P61-YES
• Proto-oncogene c-Yes
• proto-oncogene tyrosine-protein kinase Yes
• v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
• Yamaguchi sarcoma oncogene
• Yes
• YES1

Background

Yes is the cellular homolog of the oncogenic protein encoded by the Yamaguchi 73 and Esh avian sarcoma viruses. After Src, Yes is the most widely expressed member of the Src family of nonreceptor tyrosine kinases. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YES1
• Uniprot:
  • P07947.3()