Fyn Antibody (7Z9F2)

Western Blot: Fyn Antibody (7Z9F2) [NBP3-16773] - Western blot analysis of extracts of various cell lines, using Fyn Rabbit mAb (NBP3-16773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 7Z9F2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Fyn Antibody (7Z9F2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Fyn (P06241). MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDD
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FYN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Fyn Antibody (7Z9F2)

• EC 2.7.10
• EC 2.7.10.2
• FYN oncogene related to SRC, FGR, YES
• Fyn
• MGC45350
• OKT3-induced calcium influx regulator
• p59-Fyn
• protein-tyrosine kinase fyn
• Proto-oncogene c-Fyn
• Proto-oncogene Syn
• proto-oncogene tyrosine-protein kinase fyn
• SLK
• SLKc-syn protooncogene
• src/yes-related novel
• Src-like kinase
• SYN
• tyrosine kinase p59fyn(T)
• tyrosine-protein kinase Fyn

Background

Fyn is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FYN