Fumarase Antibody

Images

 
Western Blot: Fumarase Antibody [NB100-61663] - Analysis of Fumarase antibody using this antibody at 0.01 ug/ml in Human Kidney lysate (35ug protein in RIPA buffer).
Western Blot: Fumarase Antibody [NB100-61663] - HEK293 overexpressing Fumarase and probed with (mock transfection in first lane).
Sandwich ELISA: Fumarase Antibody [NB100-61663] - (1.5ug/ml) as the reporter with another as the capture rabbit antibody (2ug/ml).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA, S-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Fumarase Antibody Summary

Immunogen
Peptide with sequence C-HPNDHVNKSQSSND corresponding to internal region according to NP_000134.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Sandwich ELISA 1 - 2 ug/ml
Application Notes
EIA: Sandwich ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. WB: Approx. 48 kDa band observed in human kidney, liver and lung lysates (calculated MW of 54.6 kDa band according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.
Positive Control
Fumarase Lysate (NBL1-10713)
Publications
Read Publication using NB100-61663.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fumarase Antibody

  • EC 4.2.1.2
  • fumarase
  • fumarate hydratase
  • fumarate hydratase, mitochondrial
  • HLRCC
  • LRCC
  • MCL
  • MCUL1

Background

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fumarase Antibody (NB100-61663)(1)

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FH
Entrez
Uniprot