Fumarase Antibody (3F6) - BSA Free

Western Blot: Fumarase Antibody (3F6) [NBP2-59442] - Lane 1: HeLa cell lysates, Lane 2: LnCaP cell lysates, Lane 3: A549 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: 293T cell lysates, Lane 6: K562 cell lysates, Lane 7: Jurkat cell lysates

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 3F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Fumarase Antibody (3F6) - BSA Free Summary

• Description: Novus Biologicals Mouse Fumarase Antibody (3F6) - BSA Free (NBP2-59442) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-Fumarase Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Fumarase (44-510aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10-1:1048
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Fumarase Antibody (3F6) - BSA Free

• EC 4.2.1.2
• fumarase
• fumarate hydratase
• fumarate hydratase, mitochondrial
• HLRCC
• LRCC
• MCL
• MCUL1

Background

Fumarase is encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fumarase Antibody (NBP2-59442)(1)

Publication using NBP2-59442

• Tan HWS, Lu G, Dong H et al. A degradative to secretory autophagy switch mediates mitochondria clearance in the absence of the mATG8-conjugation machinery Nature communications 2022-06-28 [PMID: 35764633]

Bioinformatics

• Gene Symbol: FH