FUBP1 Antibody (SY11-04)

Western Blot: FUBP1 Antibody (SY11-04) [NBP2-67386] - Analysis of FUBP1 on different lysates using anti-FUBP1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Raji

Immunocytochemistry/Immunofluorescence: FUBP1 Antibody (SY11-04) [NBP2-67386] - Staining FUBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: FUBP1 Antibody (SY11-04) [NBP2-67386] - Analysis of paraffin-embedded mouse stomach tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.

Flow Cytometry: FUBP1 Antibody (SY11-04) [NBP2-67386] - Flow cytometric analysis of Jurkat cells with FUBP1 antibody at 1/50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunocytochemistry/Immunofluorescence: FUBP1 Antibody (SY11-04) [NBP2-67386] - Staining FUBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: FUBP1 Antibody (SY11-04) [NBP2-67386] - Staining FUBP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: FUBP1 Antibody (SY11-04) [NBP2-67386] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FUBP1 Antibody (SY11-04) [NBP2-67386] - Analysis of paraffin-embedded human pancreas tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FUBP1 Antibody (SY11-04) [NBP2-67386] - Analysis of paraffin-embedded human tonsil tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FUBP1 Antibody (SY11-04) [NBP2-67386] - Analysis of paraffin-embedded mouse brain tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FUBP1 Antibody (SY11-04) [NBP2-67386] - Analysis of paraffin-embedded mouse pancreas tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.

Product Discontinued

FUBP1 Antibody (SY11-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human FUBP1 aa 584-633 / 644. (SwissProt: Q96AE4 Human; SwissProt: Q91WJ8 Mouse; SwissProt: Q32PX7 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FUBP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for FUBP1 Antibody (SY11-04)

• far upstream element (FUSE) binding protein 1
• far upstream element binding protein
• far upstream element-binding protein 1
• FBPDNA helicase V
• FUBP
• FUSE-binding protein 1
• hDH V

Background

FUBP1 encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FUBP1