FTO Antibody - BSA Free

Western Blot: FTO Antibody [NBP1-77021] - Analysis of FTO in human uterus tissue lysate with FTO antibody at 1 ug/mL.

Immunohistochemistry: FTO Antibody - BSA Free [NBP1-77021] - Immunohistochemistry of FTO in mouse brain tissue with FTO antibody at 2.5 u/mL.

Immunocytochemistry/ Immunofluorescence: FTO Antibody - BSA Free [NBP1-77021] - Immunofluorescence of FTO in Mouse Brain cells with FTO antibody at 20 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

FTO Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FTO Antibody - BSA Free (NBP1-77021) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-FTO Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FTO. The immunogen is located within the first 50 amino acids of FTO. Amino Acid Squence: TWLPYLTPKDDEFYQ
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FTO
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  FTO Peptide (NBP1-77021PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for FTO Antibody - BSA Free

• Alpha-ketoglutarate-dependent dioxygenase FTO
• EC 1.14.11.-
• fat mass and obesity associated
• Fat mass and obesity-associated protein
• FTO
• KIAA1752protein fto
• MGC5149

Background

Rising obesity rates are rapidly becoming a growing health concern in the developing world. The fat mass and obesity associated gene (FTO) is the first gene discovered to contribute to common forms of human obesity. FTO is a member of the non-heme dioxygenase superfamily, encoding a 2-oxoglutarate-dependent nucleic acid demethylase whose mRNA is widely expressed, especially in neurons of feeding-related nuclei of the brain. FTO mRNA in the arcuate nucleus in mice is up-regulated by feeding and down-regulated during fasting, although the opposite pattern has been observed in rats. At least four isoforms of FTO are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FTO Antibody (NBP1-77021)(1)

Publication using NBP1-77021

• W Aline Ingelson-Filpula, Karen L Kadamani, Mohammad Ojaghi, Matthew E Pamenter, Kenneth B Storey Hypoxia-induced downregulation of RNA m 6 A protein machinery in the naked mole-rat heart. Biochimie 2024-05-22 [PMID: 38788827]

FAQs for FTO Antibody (NBP1-77021). (Showing 1 - 1 of 1 FAQ).

1. Do you have any antibodies against FTO, where you can indicate the sequence of the immunogen?
   • With regards to other FTO antibodies that we do list the peptide sequence for, the immunogen sequence for catalog number NB110-60935 can be found in the range of amino acids 400-505. Many of the exact immunogen sequences used for our products are proprietary and cannot be disclosed. In these instances we try to provide a reasonable amino acid range to help in your decision.

Bioinformatics

• Gene Symbol: FTO
• Uniprot:
  • Q9C0B1(Human)