FSHR Antibody (626717) [Unconjugated]

F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins." > F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins." title="HEK293 human embryonic kidney cell line transfected with (A) human FSH R or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591) followed by APC-conjugated Goat anti-Mouse Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins." />

HEK293 human embryonic kidney cell line transfected with (A) human FSH R or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591) followed by APC-conjugated Goat anti-Mouse Secondary Antibody (Catalog # <a class=

5925-FS) induces cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R in a dose-dependent manner (orange line), as measured by the cAMP Parameter Assay Kit (Catalog # KGE002B). cAMP production elicited by Recom-binant Human FSH a/ beta (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591). The ND₅₀ is typically 0.02-0.1 µg/mL." > 5925-FS) induces cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R in a dose-dependent manner (orange line), as measured by the cAMP Parameter Assay Kit (Catalog # KGE002B). cAMP production elicited by Recom-binant Human FSH a/ beta (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591). The ND₅₀ is typically 0.02-0.1 µg/mL." title="Recombinant Human FSH a/ beta (Catalog # 5925-FS) induces cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R in a dose-dependent manner (orange line), as measured by the cAMP Parameter Assay Kit (Catalog # KGE002B). cAMP production elicited by Recom-binant Human FSH a/ beta (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591). The ND₅₀ is typically 0.02-0.1 µg/mL." />

Recombinant Human FSH a/ beta (Catalog # <a class=

• Applications: Flow, B/N, CyTOF-ready
• Clone: 626717
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FSHR Antibody (626717) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human FSH R
  Accession # M65085
• Specificity: Detects human FSH R in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FSHR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.02-0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FSHR Antibody (626717) [Unconjugated]

• follicle stimulating hormone receptor
• follicle-stimulating hormone receptor
• Follitropin receptor
• FSH R
• FSH receptor
• FSHR
• FSH-R
• LGR1
• LGR1FSHRO
• MGC141667
• MGC141668
• ODG1

Background

Follicle stimulating hormone receptor (FSH R), also called follitropin receptor or LGR1, is a 695 amino acid (aa), ~87 kDa 7-transmembrane receptor of the leucine-rich repeat glycoprotein hormone receptor subfamily of GPCRs. It is expressed by ovarian granulosa cells and is essential for ovarian follicle maturation. In the male, it is expressed by Sertoli cells and plays a minor role in male fertility. Human FSH R shares 86% aa sequence identity with mouse and rat FSH R within the 349 aa N‑terminal extracellular domain. A 754 aa isoform contains an inserted sequence at aa 75.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FSHR Antibody (MAB65591)(4)

Publications using MAB65591

• Guohua Song, Yadong Wang, Yaling Wang, Yixuan Jiang, Shuaijie Sun, Hanfang Cai et al. Coprophagy Prevention Decreases the Reproductive Performance and Granulosa Cell Apoptosis via Regulation of CTSB Gene in Rabbits Frontiers in Physiology 2022-07-18 [PMID: 35923240]
• Guohua Song, Yixuan Jiang, Yaling Wang, Mingkun Song, Xuanmin Niu, Huifen Xu et al. Modulation of Cathepsin S (CTSS) Regulates the Secretion of Progesterone and Estradiol, Proliferation, and Apoptosis of Ovarian Granulosa Cells in Rabbits Animals (Basel) 2021-06-13 [PMID: 34199180] (Immunohistochemistry, Rabbit)
• F Wang, AY Wang, C Chesnelong, Y Yang, A Nabbi, S Thalappill, V Alekseev, K Riabowol ING5 activity in self-renewal of glioblastoma stem cells via calcium and follicle stimulating hormone pathways Oncogene, 2017-09-18;37(3):286-301. 2017-09-18 [PMID: 28925404] (ICC, Human)
• Dongzhi Yang, Liangzhu Feng, Casey A. Dougherty, Kathryn E. Luker, Daiqin Chen, Meagan A. Cauble et al. In vivo targeting of metastatic breast cancer via tumor vasculature-specific nano-graphene oxide Biomaterials 2016-10-01 [PMID: 27490486]

Bioinformatics

• Gene Symbol: FSHR
• Uniprot:
  • M65085()