FRS2 Overexpression Lysate

Images

 
Western Blot: FRS2 Overexpression Lysate (Adult Normal) [NBL1-10836] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FRS2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FRS2 Overexpression Lysate Summary

Description

FRS2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC205842

Accession#: NM_006654

Protein Tag: C-MYC/DDK
Gene
FRS2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
56.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FRS2 Overexpression Lysate

  • FGFR signalling adaptor
  • FGFR-signaling adaptor SNT
  • fibroblast growth factor receptor substrate 2
  • FRS2
  • FRS2A
  • FRS2alpha
  • SNT
  • SNT1
  • SNT-1FGFR substrate 2
  • Suc1-associated neurotrophic factor target 1

Background

FGFs (Fibroblast Growth Factors) bind to FGF receptors (FGFRs) monovalently, and FGF receptor dimerization and activation is mediated by multivalent interactions between heparin sulfate proteoglycans and FGF. Upon activation, receptor tyrosine kinases undergo rapid autophosphorylation on numerous tyrosine residues. Autophosphorylation sites located within the catalytic domain are crucial for stimulation of kinase activity, while autophosphorylation sites located in other regions are usually involved in the recruitment of cellular target proteins. Fibroblast growth factor or nerve growth factor (NGF) stimulation leads to tyrosine phosphorylation of the FGF Receptor Substrate 2 (FRS2) docking proteins. FRS2 is also designated SNT (Suc-1 associated neurotrophic factor target protein). Two structurally similar forms of FRS2 are: FRS2a (SNT-1) and FRS-2b (SNT-2, FRS3). FRS functions as a lipid-anchored docking protein that is tyrosine phosphorylated and recruited to FGFR upon FGF stimulation. FRS2 plays an important role in linking FGF and NGF with the Ras/MAPK signaling pathway, thus relaying information from the cell surface to the nucleus. FRS2 contains both a consensus myristylation sequence, involved in its recruitment to the cell membrane and a putative phosphotyrosine binding (PTB)domain in its amino-terminus. The interaction of FRS2 with FGFR1 occurs via the PTB domain of FRS2 binding to a 12-residue segment in the juxtamembrane region of FGFR1. FRS2a contains four binding sites for the adaptor protein Grb2 and two binding sites for the protein tyrosine phosphatase Shp2. FGF stimulation leads to phosphorylation of Shp2 on a tyrosine residue forming a complex with an additional molecule of Grb2. Grb2/Sos complexes are thus recruited directly and indirectly via Shp2 upon tyrosine phosphorylation of FRS2a in response to growth factor stimulation. It has been shown that FRS2 proteins are tyrosine phos phorylated in response to activation of the RET receptor, a tyrosine kinase that functions as the signal transducing receptor for the GDNF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol FRS2