FRS2 [p Tyr436] Antibody

Images

 
Western blot shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes and PC‑12 rat adrenal pheochromocytoma cell line untreated or treated with ...read more
FRS2 phosphorylated at Y436 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

FRS2 [p Tyr436] Antibody Summary

Immunogen
Phosphopeptide containing human FRS2 Y436 site
Modification
p Tyr436
Specificity
Detects human, mouse, and rat FRS2 isoforms when phosphorylated at Y436.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FRS2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5126 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FRS2 [p Tyr436] Antibody

  • FGFR signalling adaptor
  • FGFR-signaling adaptor SNT
  • fibroblast growth factor receptor substrate 2
  • FRS2
  • FRS2A
  • FRS2alpha
  • SNT
  • SNT1
  • SNT-1FGFR substrate 2
  • Suc1-associated neurotrophic factor target 1

Background

FRS2 (FGF R substrate 2; also known as SNT and FRS2-alpha ) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF and RTK receptors and their Ras/MAPK signaling cascades. FRS2 contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain that interacts with FGF and NGF receptors (aa 13‑115), and a C-terminal tyrosine-rich region that serves as a docking site for GRB-2 and SHP-2 (aa 196‑471). Phosphorylation of Y436 by activated RTKs is required for efficient SHP-2 recruitment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FRS2 Antibody (AF5126)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC-P, Neutralization, Western Blot.


Filter By Application
IHC-P
(3)
Neutralization
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(5)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF5126 Applications Species
PCC Liu, H Koblish, L Wu, K Bowman, S Diamond, D DiMatteo, Y Zhang, M Hansbury, M Rupar, X Wen, P Collier, P Feldman, R Klabe, KA Burke, M Soloviev, C Gardiner, X He, A Volgina, M Covington, B Ruggeri, R Wynn, TC Burn, P Scherle, S Yeleswaram, W Yao, R Huber, G Hollis INCB054828 (pemigatinib), a potent and selective inhibitor of fibroblast growth factor receptors 1, 2, and 3, displays activity against genetically defined tumor models PLoS ONE, 2020;15(4):e0231877. 2020 [PMID: 32315352] (Western Blot, Mouse) Western Blot Mouse
L Xu, X Meng, N Xu, W Fu, H Tan, L Zhang, Q Zhou, J Qian, S Tu, X Li, Y Lao, H Xu Gambogenic acid inhibits fibroblast growth factor receptor signaling pathway in erlotinib-resistant non-small-cell lung cancer and suppresses patient-derived xenograft growth Cell Death Dis, 2018;9(3):262. 2018 [PMID: 29449529] (Western Blot, Human) Western Blot Human
Jones A, Teschendorff A, Li Q, Hayward J, Kannan A, Mould T, West J, Zikan M, Cibula D, Fiegl H, Lee S, Wik E, Hadwin R, Arora R, Lemech C, Turunen H, Pakarinen P, Jacobs I, Salvesen H, Bagchi M, Bagchi I, Widschwendter M Role of DNA methylation and epigenetic silencing of HAND2 in endometrial cancer development. PLoS Med, 2013;10(11):e1001551. 2013 [PMID: 24265601] (IHC-P, Mouse) IHC-P Mouse
Bohrer , Laura R, Chuntova , Pavlina, Bade , Lindsey, Beadnell , Thomas C, Leon , Ronald P, Brady , Nicholas, Ryu , Yungil, Goldberg , Jodi E, Schmechel , Stephen, Koopmeiners , Joseph S, McCarthy , James B, Schwertfeger , Kathryn Activation of the FGFR-STAT3 pathway in breast cancer cells induces a hyaluronan-rich microenvironment that licenses tumor formation. Cancer Res, 2014;74(1):374-86. 2014 [PMID: 24197137] (Neutralization, Mouse) Neutralization Mouse
Nallasamy S, Li Q, Bagchi MK, Bagchi IC Msx homeobox genes critically regulate embryo implantation by controlling paracrine signaling between uterine stroma and epithelium. PLoS Genet., 2012;8(2):e1002500. 2012 [PMID: 22383889] (IHC-P, Mouse) IHC-P Mouse
Garcia CM, Huang J, Madakashira BP, Liu Y, Rajagopal R, Dattilo L, Robinson ML, Beebe DC The function of FGF signaling in the lens placode. Dev. Biol., 2011;351(1):176-85. 2011 [PMID: 21223962] (IHC-P, Mouse) IHC-P Mouse

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Bioinformatics

Gene Symbol FRS2