Frizzled-8 Antibody [HRP]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP

Frizzled-8 Antibody [HRP] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-8
  Ala25-Pro173
  Accession # Q61091
• Specificity: Detects mouse Frizzled-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Frizzled-9 is observed and less than 1% cross-reactivity with rmFrizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, rmFrizzled-6, and rmFrizzled‑7 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FZD8
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Frizzled-8 Antibody [HRP]

• frizzled (Drosophila) homolog 8
• frizzled 8
• frizzled family receptor 8
• frizzled homolog 8 (Drosophila)
• Frizzled8
• Frizzled-8
• FZ-8
• FZD8
• hFZ8

Background

"Frizzled-8 is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FZD8