Frizzled-6 Antibody

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Frizzled-6 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-6
  His19-Val153
  Accession # Q61089
• Specificity: Detects mouse Frizzled-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Frizzled-6 is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, rmFrizzled-7, rmFrizzled-8, and rmFrizzled-9 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FZD6
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-6 Antibody

• frizzled (Drosophila) homolog 6
• frizzled homolog 6 (Drosophila)
• Frizzled6
• Frizzled-6
• FZ6
• fz-6
• FZD6
• Hfz6
• seven transmembrane helix receptor

Background

Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Frizzled-6 Antibody (AF1526)(9)

Publications using AF1526

• Y Ozono, A Tamura, S Nakayama, E Herawati, Y Hanada, K Ohata, M Takagishi, M Takahashi, T Imai, Y Ohta, K Oshima, T Sato, H Inohara, S Tsukita Daple deficiency causes hearing loss in adult mice by inducing defects in cochlear stereocilia and apical microtubules Scientific Reports, 2021;11(1):20224. 2021-01-01 [PMID: 34642354] (IHC, Mouse)
• KS Kindt, A Akturk, A Jarysta, M Day, A Beirl, M Flonard, B Tarchini EMX2-GPR156-G&alphai reverses hair cell orientation in mechanosensory epithelia Nature Communications, 2021;12(1):2861. 2021-01-01 [PMID: 34001891] (IHC, Mouse)
• L George, A Mitra, TA Thimraj, M Irmler, S Vishweswar, L Lunding, D Hühn, A Madurga, J Beckers, H Fehrenbach, S Upadhyay, H Schulz, GD Leikauf, K Ganguly Transcriptomic analysis comparing mouse strains with extreme total lung capacities identifies novel candidate genes for pulmonary function Respir. Res., 2017;18(1):152. 2017-01-01 [PMID: 28793908] (IHC-P, Mouse)
• J Wen, H Tao, K Lau, H Liu, CA Simmons, Y Sun, S Hopyan Cell and Tissue Scale Forces Coregulate Fgfr2-Dependent Tetrads and Rosettes in the Mouse Embryo Biophys. J., 2017;112(10):2209-2218. 2017-01-01 [PMID: 28538157] (IHC, Mouse)
• F Oozeer, LL Yates, C Dean, CJ Formstone A role for core planar polarity proteins in cell contact-mediated orientation of planar cell division across the mammalian embryonic skin Sci Rep, 2017;7(1):1880. 2017-01-01 [PMID: 28500339] (IHC, Mouse)
• Chang H, Smallwood P, Williams J, Nathans J The spatio-temporal domains of Frizzled6 action in planar polarity control of hair follicle orientation. Dev Biol, 2016;409(1):181-93. 2016-01-01 [PMID: 26517967] (IHC-Fr, Mouse)
• Lau K, Tao H, Liu H, Wen J, Sturgeon K, Sorfazlian N, Lazic S, Burrows J, Wong M, Li D, Deimling S, Ciruna B, Scott I, Simmons C, Henkelman R, Williams T, Hadjantonakis A, Fernandez-Gonzalez R, Sun Y, Hopyan S Anisotropic stress orients remodelling of mammalian limb bud ectoderm. Nat Cell Biol, 2015;17(5):569-79. 2015-01-01 [PMID: 25893915] (IHC, Mouse)
• Li , Xiaofei, Xu , Lei, Li , Jianfeng, Li , Boqin, Bai , Xiaohui, Strauss , Jerome F, Zhang , Zhibing, Wang , Haibo Otitis media in sperm-associated antigen 6 (Spag6)-deficient mice. PLoS ONE, 2014;9(11):e112879. 2014-01-01 [PMID: 25393619] (IHC, Mouse)
• Webb SW, Grillet N, Andrade LR, Xiong W, Swarthout L, Della Santina CC, Kachar B, Muller U Regulation of PCDH15 function in mechanosensory hair cells by alternative splicing of the cytoplasmic domain. Development, 2011;138(8):1607-17. 2011-01-01 [PMID: 21427143] (IHC, Mouse)

Bioinformatics

• Gene Symbol: FZD6
• Uniprot:
  • Q61089()