Frizzled-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NLS3488] - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NLS3488] - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry: Frizzled-2 Antibody - BSA Free [NLS3488] - Immunohistochemistry of Lung, Non Small-Cell Carcinoma

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Ha, Sh
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Frizzled-2 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 18 amino acid peptide from 3rd extracellular domain of human Frizzled-2.
• Epitope: 3rd extracellular domain
• Localization: Integral membrane protein
• Specificity: Human Frizzled-2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD7 (67%), OR1A2 (56%), NFATC2 (50%).
• Predicted Species: Sheep (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FZD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10 ug/ml
• Application Notes: .

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Opossum (89%), Turkey (83%), Chicken (83%), Xenopus (83%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Frizzled-2 Antibody - BSA Free

• frizzled (Drosophila) homolog 2
• frizzled homolog 2 (Drosophila)
• Frizzled2
• Frizzled-2
• Fz2
• fz-2
• FZD2
• fzE2
• hFz2

Background

FZD2 is a Frizzled Receptor that mediates Wnt signaling, which is required for proper axis development in the embryo. FZD2 is developmentally regulated. Studies of rat FZD2 indicate that the protein signals through a calcium-releasing pathway and that FDZ2 expression modulates protein kinase C (PKC) localization and stimulates PKC activity in vitro. Increased levels of FZD2 mRNA have been observed in primary gastric cancer and in head and neck squamous carcinoma and gastric cancer cell lines. FZD2 expression has been documented in human brain, heart, kidney, lung, colon, ovary and stomach. High levels of expression have been observed in fetal kidney and lung and adult colon and ovary. ESTs have been isolated from human tissue libraries, including cancerous kidney, lung, lymph and testis, and normal blood, cervix, embryo and kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Frizzled-2 Antibody (NLS3488)(1)

Publication using NLS3488

• Metsuyanim S, Harari-Steinberg O, Buzhor E et al. Expression of stem cell markers in the human fetal kidney. PLoS One 2009-01-01 [PMID: 19696931]

Bioinformatics

• Gene Symbol: FZD2
• Entrez:
  • 2535(Human)
  • 57265(Mouse)