FoxO1/FKHR Antibody

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Western Blot: FoxO1/FKHR Antibody [NB100-2312] - Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-FOXO1a antibody used for WB ...read more
Independent Antibodies: Immunoprecipitation: FoxO1/FKHR Antibody [NB100-2312] - Detection of human FOXO1a by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

FoxO1/FKHR Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 600 and the C-terminus (residue 652) of human Forkhead Box O1a using the numbering given in Swiss-Prot entry Q12778 (GeneID 2308).
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000- 1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 2-10 ug/mg lysate
Application Notes
Use in ICC/IF reported in scientific literature (PMID 25344740)
Control
FoxO1/FKHR Overexpression Lysate
Publications
Read Publications using
NB100-2312 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28467929).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FoxO1/FKHR Antibody

  • FKH1
  • FKHR
  • FKHRforkhead box protein O1
  • forkhead box O1
  • Forkhead box protein O1A
  • forkhead homolog in rhabdomyosarcoma
  • Forkhead in rhabdomyosarcoma
  • FoxO1
  • FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxO1/FKHR Antibody (NB100-2312)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-2312 Applications Species
Calvier L, Chouvarine P, Legchenko E et al. PPARy Links BMP2 and TGFb1 Pathways in Vascular Smooth Muscle Cells, Regulating Cell Proliferation and Glucose Metabolism. Cell Metab. May 2 2017 [PMID: 28467929] (Mouse) Mouse
Savai R, Al-Tamari Hm, Sedding D et al. Pro-proliferative and inflammatory signaling converge on FoxO1 transcription factor in pulmonary hypertension. Nat. Med. 2014 Oct 26 [PMID: 25344740] (WB, ICC/IF, Human) WB, ICC/IF Human
van der Horst PH, Wang Y, Vandenput I et al. Progesterone inhibits epithelial-to-mesenchymal transition in endometrial cancer. PLoS One 2012 [PMID: 22295114]
Yin X, Pavone ME, Lu Z et al. Increased activation of the PI3K/AKT pathway compromises decidualization of stromal cells from endometriosis. J Clin Endocrinol Metab 2012 Jan [PMID: 22072736]
Khatri S, Yepiskoposyan H, Gallo CA et al. FOXO3a regulates glycolysis via transcriptional control of tumor suppressor TSC1. J Biol Chem 2010 May [PMID: 20371605]
Amin AR, Thakur VS, Gupta K et al. Restoration of p53 functions protects cells from concanavalin A-induced apoptosis. Mol Cancer Ther 2010 Feb [PMID: 20124456]
Hoekstra AV, Sefton EC, Berry E et al. Progestins activate the AKT pathway in leiomyoma cells and promote survival. J Clin Endocrinol Metab 2009 May [PMID: 19240153]
Ward EC, Hoekstra AV, Blok LJ et al. The regulation and function of the forkhead transcription factor, Forkhead box O1, is dependent on the progesterone receptor in endometrial carcinoma. Endocrinology 2008 Apr [PMID: 18096667]
Liu Z, Du J, Ren J et al. miR-183-96-182 clusters alleviated ox-LDL-induced vascular endothelial cell apoptosis in vitro by targeting FOXO1 RSC Advances 2018 Oct 12 (WB, Human) WB Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxO1/FKHR Antibody (NB100-2312). (Showing 1 - 1 of 1 FAQs).

  1. I've bought 2 antibodies from novus biologicals (NB100-614, NB100-2312) and now i am optimizing them for IP. Which beads did you use, protein a or protein g beads? in some cases only one kind of beads work, i have now tried protein a beads. If possible it would be also very helpful which protocol you used for the IP? (buffers, volumes, incubation times?)
    • Protein A beads were used for IP for these products. We don't have a specific protocol. We recommend for NB100-2312 a dilution of 1 to 10 mcg/mg lysate. For NB100-614 the dilution is 2 to 5 ug/mg lysate. Please see our general IP protocol.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for FoxO1/FKHR Antibody (NB100-2312)

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Blogs on FoxO1/FKHR.

FOXO1/FKHR (fork head in Rhabdomyosarcoma)
FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,...  Read full blog post.

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Bioinformatics

Gene Symbol FOXO1
Entrez
Uniprot