FoxO1/FKHR Antibody (3B6)

Images

 
Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Western blot analysis using FOXO1A mouse mAb against Hela (1), HEK293 (2), MCF-7(3), and C6 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Immunofluorescence analysis of Hela cells using FOXO1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Immunohistochemical analysis of paraffin-embedded intima cancer tissues using FOXO1A mouse mAb with DAB staining.
Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Western blot analysis using FOXO1A mAb against human FOXO1 (AA: 471-600) recombinant protein. (Expected MW is 39.3 kDa)
ELISA: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
3B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

FoxO1/FKHR Antibody (3B6) Summary

Immunogen
Purified recombinant fragment of human FOXO1A expressed in E. Coli.
Localization
Cytoplasm
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOXO1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
69.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-22190 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for FoxO1/FKHR Antibody (3B6)

  • FKH1
  • FKHR
  • FKHRforkhead box protein O1
  • forkhead box O1
  • Forkhead box protein O1A
  • forkhead homolog in rhabdomyosarcoma
  • Forkhead in rhabdomyosarcoma
  • FoxO1
  • FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxO1/FKHR Antibody (NBP2-22190) (0)

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Review for FoxO1/FKHR Antibody (NBP2-22190) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-22190:
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Images Ratings Applications Species Date Details
Western Blot FoxO1/FKHR NBP2-22190
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reviewed by:
Anonymous
WB Human 08/23/2015
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Summary

ApplicationWestern Blot
Sample TestedNuclear fraction of human breast cancer cell lines
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionSheep anti-mouse IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA931V
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxO1/FKHR Antibody (NBP2-22190) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for FoxO1/FKHR Antibody (NBP2-22190)

Discover related pathways, diseases and genes to FoxO1/FKHR Antibody (NBP2-22190). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for FoxO1/FKHR Antibody (NBP2-22190)

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Research Areas for FoxO1/FKHR Antibody (NBP2-22190)

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Blogs on FoxO1/FKHR.

FOXO1/FKHR (fork head in Rhabdomyosarcoma)
FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,...  Read full blog post.

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Recent Reviews

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1
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2
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1
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Anonymous
08/23/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol FOXO1
Entrez