FoxM1 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Biotin
Concentration
LYOPH

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FoxM1 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human FoxM1
Thr642-Gln763
Accession # Q08050
Specificity
Detects human FoxM1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) FoxJ1 is observed and less than 1% cross-reactivity with rhFoxB2, rhFoxC2, rhFoxD3, rhFoxF1, rhFoxH1, rhFoxO3A, rhFoxP1, and rhFoxP3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
FOXM1
Purity
Antigen Affinity-purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxM1 Antibody [Biotin]

  • FKHL16
  • FKHL16trident
  • forkhead box M1
  • forkhead box protein M1
  • Forkhead, drosophila, homolog-like 16
  • forkhead-like 16
  • Forkhead-related protein FKHL16
  • FoxM1
  • Hepatocyte nuclear factor 3 forkhead homolog 11
  • HFH-11
  • HFH11FOXM1B
  • HFH-11M-phase phosphoprotein 2
  • HNF-3
  • HNF-3/fork-head homolog 11
  • INS-1
  • MPHOSPH2
  • MPP-2
  • MPP2MPM-2 reactive phosphoprotein 2
  • PIG29
  • TGT3
  • Transcription factor Trident
  • Trident
  • WIN
  • Winged-helix factor from INS-1 cells

Background

FoxM1 (Forkhead box protein M1; also FKHL16, HFH11 and WIN) is a 90‑100 kDa member of the winged helix transcription factor gene family. It is expressed in proliferating and tumor cells and promotes the transition from G1 to S phase and G2 to M phase within the cell cycle. Human FoxM1 is 763 amino acids (aa) in length. It contains three PEST sequences (aa 22‑34, 460‑472 and 478‑515), a forkhead DNA binding domain (aa 235‑327), a Pro/Ser/Thr-rich region (aa 480‑513) and a phosphorylation site at Ser704. There are at least two splice variants. The first shows a 38 aa insertion after Val423. This blocks FoxM1 transcriptional activation activity. The second variant shows a deletion of aa 326‑340. Over aa 642‑763, human FoxM1 shares 73% aa identity with mouse FoxM1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF3975

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Bioinformatics

Gene Symbol FOXM1
Uniprot