FoxM1 Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

FoxM1 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human FoxM1
  Thr642-Gln763
  Accession # Q08050
• Specificity: Detects human FoxM1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxB2 and rhFoxJ1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxM1 Antibody [Alexa Fluor® 700]

• FKHL16
• FKHL16trident
• forkhead box M1
• forkhead box protein M1
• Forkhead, drosophila, homolog-like 16
• forkhead-like 16
• Forkhead-related protein FKHL16
• FoxM1
• Hepatocyte nuclear factor 3 forkhead homolog 11
• HFH-11
• HFH11FOXM1B
• HFH-11M-phase phosphoprotein 2
• HNF-3
• HNF-3/fork-head homolog 11
• INS-1
• MPHOSPH2
• MPP-2
• MPP2MPM-2 reactive phosphoprotein 2
• PIG29
• TGT3
• Transcription factor Trident
• Trident
• WIN
• Winged-helix factor from INS-1 cells

Background

FoxM1 (Forkhead box protein M1; also FKHL16, HFH11 and WIN) is a 90-100 kDa member of the winged helix transcription factor gene family. It is expressed in proliferating and tumor cells and promotes the transition from G₁ to S phase and G₂ to M phase within the cell cycle. Human FoxM1 is 763 amino acids (aa) in length. It contains three PEST sequences (aa 22-34, 460-472 and 478-515), a forkhead DNA binding domain (aa 235-327), a Pro/Ser/Thr-rich region (aa 480-513) and a phosphorylation site at Ser704. There are at least two splice variants. The first shows a 38 aa insertion after Val423. This blocks FoxM1 transcriptional activation activity. The second variant shows a deletion of aa 326-340. Over aa 642-763, human FoxM1 shares 73% aa identity with mouse FoxM1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.