FoxK1 Antibody - BSA Free

Western Blot: FoxK1 Antibody [NB100-88145] - Immunoprecipitates. Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-FoxK1 used for IP at 3 mcg/mg lysate. FoxK1 was also immunoprecipitated by another rabbit anti- FoxK1 antibody which recognizes a downstream epitope.

Immunohistochemistry: FoxK1 Antibody [NB100-88145] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-FOXK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FoxK1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FoxK1 Antibody - BSA Free (NB100-88145) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 525 and 575 of human forkhead box K1 using the numbering given in entry NP_001032242.1 (GeneID 221937).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FOXK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2 -5 ug/mg lysate
  • Western Blot
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FoxK1 Antibody - BSA Free

• FLJ14977
• forkhead box K1
• forkhead box protein K1
• FoxK1
• FOXK1L
• IMAGE:5164497
• MNF
• Myocyte nuclear factor

Background

Forkhead box protein K1 (FoxK1) is a member of the human Forkhead-box (FOX) family of proteins. The Forkhead-box domain is a highly conserved DNA binding domain of about 100 amino acids found in proteins that function as transcriptional regulators. FoxK1 also bears an FHA (forkhead-associated) domain, a putative nuclear signaling domain that binds phospho-peptides. FoxK1 has been shown to play a role in the regulation of myogeneic progenitor cells and skeletal muscle regeneration. FoxK1 is also known as myocyte nuclear factor, MNF, FOXK1L, and FLJ14977.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXK1
• Entrez:
  • 221937(Human)
• Uniprot:
  • NP_001032242.1(Human)