ILF1 Antibody - BSA Free

Western Blot: ILF1 Antibody [NBP1-87700] - Whole cell lysates from SUM159, MDA-MB-231, MCF-7 and HCC1954 cells were loaded with 50 ug/lane. 10% SDS-PAGE. ILF1 antibody (NBP1-87700) was used for primary antibody: 1:2000, 4C, overnight. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: ILF1 Antibody [NBP1-87700] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: ILF1 Antibody [NBP1-87700] - Staining of human small intestine shows distinct nuclear positivity in glandular cells.

Western Blot: ILF1 Antibody [NBP1-87700] - Analysis in human cell line RT-4 and human cell line U-251 MG.

ChIP-Exo-Seq composite graph for Anti-FOXK2 (NBP1-87700) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ILF1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ILF1 Antibody - BSA Free (NBP1-87700) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SSQPVLITVQRQLPQAIKPVTYTVATPVTTSTSQPPVVQTVHVVHQIPAVSVTSVAGLAPANTYTVSGQAVVTPAAVLAPPKAEAQENGDHREVKVKVEPIPAIGHATLGTASRIIQTAQTTPVQTVTIVQQAPLGQHQLPIK
• Predicted Species: Mouse (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FOXK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq 1-10ug per reaction
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ILF1 Protein (NBP1-87700PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ILF1 Antibody - BSA Free

• Cellular transcription factor ILF-1
• forkhead box K2
• forkhead box protein K2
• FOXK1
• ILF-1
• ILF1ILF
• interleukin enhancer binding factor 1
• Interleukin enhancer-binding factor 1

Background

ILF1 is encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ILF1 Antibody (NBP1-87700)(1)

Publication using NBP1-87700

• Wu, P;Song, N;Xiang, Y;Tao, Z;Mao, B;Guo, R;Wang, X;Wu, D;Zhang, Z;Chen, X;Ma, D;Zhang, T;Hao, B;Ma, J; FOXK2 in skeletal muscle development: a new pathogenic gene for congenital myopathy with ptosis EMBO molecular medicine 2025-05-23 [PMID: 40410591]

Review for ILF1 Antibody (NBP1-87700) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 11/15/2022

Summary

• Application: Western Blot
• Sample Tested: MCF-7,MDA-MB-231,SUM-159PT
• Species: Human
• Lot: 000010450

Bioinformatics

• Gene Symbol: FOXK2