FoxJ1/HFH4 Antibody (CL3989) - Azide and BSA Free

Analysis in human fallopian tube and skeletal muscle tissues using NBP3-44120 antibody. Corresponding FoxJ1/HFH4 RNA-seq data are presented for the same tissues.

Staining of BEWO cells using the Anti-FoxJ1/HFH4 monoclonal antibody) .

Staining of human endometrium shows strong nuclear positivity in a subset of glandular cells.

Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human tonsil shows no positivity in germinal center cells as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL3989
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

FoxJ1/HFH4 Antibody (CL3989) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q92949, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXJ1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:1000 - 1:2000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for FoxJ1/HFH4 Antibody (CL3989) - Azide and BSA Free

• FKHL13
• FKHL13forkhead transcription factor HFH-4
• forkhead box J1
• forkhead-like 13
• Forkhead-related protein FKHL13
• FoxJ1
• Hepatocyte nuclear factor 3 forkhead homolog 4
• HFH-4
• HFH4fork head homologue 4
• HFH-4forkhead box protein J1
• MGC35202

Background

FOXJ1 is a member of the forkhead gene family, which was originally identified in Drosophila. The forkhead family is composed of transcription factors with a conserved 100-amino acid DNA-binding motif.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXJ1