FoxD3 Antibody (800602) [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: ICC/IF
• Clone: 800602
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

FoxD3 Antibody (800602) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant mouse FoxD3
  Met1-Val130
  Accession # Q61060
• Specificity: Detects mouse FoxD3 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FoxD3 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxD3 Antibody (800602) [Alexa Fluor® 488]

• AIS1
• CWH3
• forkhead box D3
• forkhead box protein D3
• FoxD3
• Genesis
• HFH2
• HNF3/FH transcription factor genesis

Background

FoxD3 (forkhead box protein D3; also HNF3, HFH2 and Genesis) is a 50 kDa member of the winged helix transcription factor gene family.  It is expressed in the epiblast, migrating neural crest, and motoneurons plus interneuron progenitors of the developing spinal cord.  Mouse FoxD3 is 465 amino acids (aa) in length.  It contains a forkhead DNA binding domain (aa 131-208) plus an Ala-rich region (aa 248-464) that contains a Gly-rich motif (aa 369-399).  There appears to be one splice form that shows a four aa insert after Gly261.  Over aa 1-130, mouse FoxD3 shares 97% and 79% aa identity with rat and human FoxD3, respectively.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.