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FOLR2 Antibody

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Western Blot: FOLR2 Antibody [NBP2-43654] - Human synovial fluid (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Folate Receptor beta antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: FOLR2 Antibody [NBP2-43654] - Human synovial fluid (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Folate Receptor beta antibody diluted at 1:1000. The HRP-conjugated anti-rabbit ...read more
Immunohistochemistry-Paraffin: FOLR2 Antibody [NBP2-43654] - Mouse prostate. Folate receptor beta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, Mu, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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FOLR2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Folate receptor beta. The exact sequence is proprietary.
Predicted Species
Rhesus Macaque (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOLR2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Application Notes
IHC: For FFPE sections we have used heat mediated EDTA based antigen retrieval, pH 8.0, 15min. ICC/IF Assay-dependent dilution.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (84%), Bovine (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for FOLR2 Antibody

  • beta-HFR
  • FBP
  • FBP/PL-1
  • folate receptor 2 (fetal)
  • Folate receptor 2
  • folate receptor beta
  • folate receptor, beta
  • Folate receptor, fetal/placental
  • folate-binding protein, fetal/placental
  • FOLR2
  • FR-beta
  • FR-P3
  • Placental folate-binding protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MCF‑7 human breast cancer cell line was stained with Mouse Anti‑Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Western blot shows lysates of human cortex tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FOLR1 at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. Use under non-reducing conditions only.
FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Band 3 Antibody
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Immunohistochemistry: Band 3 Antibody [NBP2-49409] - Staining of human spleen shows strong positivity in erythrocytes.
Immunohistochemistry-Paraffin: Band 3 Antibody [NBP2-49409] - Staining of human bone marrow shows high expression.
Immunohistochemistry-Paraffin: Band 3 Antibody [NBP2-49409] - Staining in human bone marrow and cerebral cortex tissues using anti-SLC4A1 antibody. Corresponding SLC4A1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
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Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Staining of human colon, liver, small intestine and spleen using Anti-BLVRB antibody NBP1-83435 (A) shows similar protein distribution across tissues to independent antibody NBP1-83434 (B).
Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Analysis using Anti-BLVRB antibody NBP1-83435 (A) shows similar pattern to independent antibody NBP1-83434 (B).
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Prolyl
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Immunocytochemistry/Immunofluorescence: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody stainin is shown in green.
Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Staining of human colon shows high expression.
Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Staining of human pancreas shows low expression as expected.
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PP14/Glycodelin
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NBP1-89781
Immunohistochemistry-Paraffin: PP14/Glycodelin Antibody [NBP1-89781] - Staining of human endometrium shows high expression.
Immunohistochemistry-Paraffin: PP14/Glycodelin Antibody [NBP1-89781] - Staining in human endometrium and prostate tissues using anti-PAEP antibody. Corresponding PAEP RNA-seq data are presented for the same tissues.
Western Blot: PP14/Glycodelin Antibody [NBP1-89781] - Analysis in human placenta tissue.
Species: Hu
Applications: IHC, IHC-P, WB
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Daudi human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Lyp Monoclonal Antibody (Catalog # MAB3428) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Lyp at approximately 108 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lyp was detected in immersion fixed paraffin-embedded sections of human lymph node using 25 µg/mL Mouse Anti-Human Lyp Monoclonal Antibody (Catalog # MAB3428) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Western Blot: CD77 Synthase Antibody [NBP1-62583] - THP-1 cell lysate, concentration 0.2-1 ug/ml.
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ASAH1 Antibody
NBP1-89296
Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Staining of human kidney shows strong granular cytoplasmic positivity in a subset of renal tubules.
Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.
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Immunocytochemistry/Immunofluorescence: FUBP1 Antibody [NBP1-83918] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.
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Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
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Western Blot: ZAK Antibody (3G5) [H00051776-M03] - ZAK monoclonal antibody (M03), clone 3G5. Western Blot analysis of ZAK expression in human stomach.
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Western Blot: SLC17A5 Antibody [NBP2-20383] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: SLC17A5 Antibody [NBP2-20383] - Sample: HepG2 cells were fixed in -20C 100% MeOH for 5 min. Green: SLC17A5 protein stained by SLC17A5 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
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NBP3-15687
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Immunohistochemistry-Paraffin: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Immunohistochemistry of paraffin-embedded mouse testis using Glycerol Kinase Rabbit mAb (NBP3-15687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Western blot analysis of extracts of various cell lines, using Glycerol Kinase (GK) antibody (NBP3-15687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
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Catalase Antibody
[Unconjugated]
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, A20 mouse B cell lymphoma cell line, andRat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Catalase at approximately 64 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. 
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Catalase at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Catalase at approximately 64 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Glucose 6
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Western Blot: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glucose 6 phosphate isomerase(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glucose 6 phosphate isomerase.
Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Glucose 6 phosphate isomerasemouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Glucose 6 phosphate isomerasemouse monoclonal antibody.
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TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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PAMR1 Antibody
AF6517
Western blot shows lysates of U2OS human osteosarcoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PAMR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6517) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PAMR1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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CDT2 Antibody
NB100-40840
Immunoprecipitation: CDT2 Antibody [NB100-40840] - Detection of human DTL/CDT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DTL/CDT2 antibody NB100-40840 (lot NB100-40840-3) used for IP at 6 ug per reaction. DTL/CDT2 was also immunoprecipitated by a previous lot of this antibody (lot NB100-40840-2). For blotting immunoprecipitated DTL/CDT2, NB100-40840 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Western Blot: CDT2 Antibody [NB100-40840] - Whole cell lysate (15 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL/CDT2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB
(1 Review)
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol FOLR2