Follistatin-related Gene Protein/FLRG/Fstl3 Antibody (210725)

• Reactivity: Mu
• Applications: WB
• Clone: 210725
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Follistatin-related Gene Protein/FLRG/Fstl3 Antibody (210725) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse FLRG
  Val24-Val256
  Accession # Q9EQC7
• Specificity: Detects mouse FLRG in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows 50-100% cross-reactivity with recombinant human FLRG.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: FSTL3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Follistatin-related Gene Protein/FLRG/Fstl3 Antibody (210725)

• FLRG
• FLRGFSRP
• follistatin-like 3 (secreted glycoprotein)
• Follistatin-like 3
• Follistatin-like protein 3
• Follistatin-related gene protein
• follistatin-related protein 3
• FSTL3

Background

Follistatin-Related Gene Protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Mouse FLRG cDNA encodes a 256 amino acid (aa) residue protein with a putative 23 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C‑terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7, and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293, and CHO cells (1-5).

1. Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.
2. Sidis, Y. et al. (2002) Endocrinology 143:1613.
3. Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.
4. Hill, J. et al. (2002) J. Biol. Chem. 277:40735.
5. Bartholin, L. et al. (2001) Oncogene 20:5409.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Follistatin-related Gene Protein/FLRG/Fstl3 Antibody (MAB1255)(1)

Publication using MAB1255

• Miron P, Lambert J, Marcil A, Cowans NJ, Stamatopoulou A, Spencer K Maternal plasma levels of follistatin-related gene protein in the first trimester of pregnancies with Down syndrome. Prenat. Diagn., 2010-03-01;30(3):224-8. 2010-03-01 [PMID: 20063262] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: FSTL3
• Uniprot:
  • Q9EQC7()