Folliculin Antibody

Images

 
Western Blot: Folliculin Antibody [NBP1-44995] - Analysis of FLCN isoforms 1, 2 and 3 on HeLa whole cell lysate
Immunocytochemistry/ Immunofluorescence: Folliculin Antibody [NBP1-44995] - Detection of FLCN (Green) in Hela cells using NBP1-44995. Nuclei (Blue) are counterstained using Hoechst 33258
Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using rabbit polyclonal Folliculin ...read more
Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human bladder cancer using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ...read more
Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using rabbit polyclonal Folliculin antibody ...read more
Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal skin from human using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ...read more
Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal liver of human using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Folliculin Antibody Summary

Immunogen
N-terminal partial recombinant human Folliculin protein [Swiss-Prot# Q8NFG4] expressed in E. coli.
Localization
Cytoplasm, Nucleus
Specificity
This antibody is expected to detect all three isoforms of Folliculin protein ( UniProt # Q8NFG4)
Predicted Species
Rat (92%), Canine (95%), Primate (99%), Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FLCN
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000
Application Notes
This Folliculin antibody is useful for Western Blot, Immunohistochemistry-Paraffin and Immunocytochemistry/Immunofluorescence applications. In WB, bands are seen at 67 kDa, 37 kDa and 26 kDa reporesenting isoforms 1, 2 and 3 of FLCN respectively.
Control
HeLa Whole Cell Lysate
Publications
Read Publication using
NBP1-44995 in the following applications:

Reactivity Notes

Human, Mouse. Immunogen sequence similarity to other species: Primate species (99%), Canine (95%), Rat (92%), Bovine (91%), Xenopus (87%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Folliculin Antibody

  • BHD skin lesion fibrofolliculoma protein
  • BHDFLCL
  • Birt-Hogg-Dube syndrome protein
  • DKFZp547A118
  • FLJ45004
  • FLJ99377
  • folliculin
  • MGC17998
  • MGC23445

Background

Folliculin (FLCN) is encoded by BHD gene (Birt-Hogg-Dube gene, altered in BHD Syndrome) and is mainly localized in the nucleus, however, has also been observed to co-localize with FLCN-interacting proteins FNIP1 and FNIP2 in the cytoplasm. Highly expressed in several tissues including skin, lung, kidney, heart, testis, stomach etc., FLCN involves in pathogenesis of proliferating epithelial fibrofolliculomas where it is overexpressed, however, its expression is significantly reduced in renal tumor tissues suggesting its tumor suppressor activity in kidneys. FLCN association with FNIP1/FNIP2 mediates its indirect interaction with AMPK subunits (PRKAA1, PRKAB1 and PRKAG1). FLCN has also been suggested to regulate phosphorylation of RPS6KB1 and implicate in energy and/or nutrient sensing through AMPK/mTOR signaling pathways. BHD knock-out mice develop cysts and renal cell tumors similar to those found in BHD patients along with low levels of phosphorylated S6 ribosomal protein. Defects in FLCN are the cause of Birt-Hogg-Dube syndrome (BHD), primary spontaneous pneumothorax (PSP), renal cell carcinoma (RCC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Folliculin Antibody (NBP1-44995)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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Product General Protocols

View specific protocols for Folliculin Antibody (NBP1-44995): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Folliculin Antibody (NBP1-44995). (Showing 1 - 1 of 1 FAQ).

  1. Could you please let me know the exact sequence of the immunogen of NBP1-44995?
    • The exact immunogen sequence for our Folliculin antibody with catalogue number NBP1-44995 is proprietary, and I am unable to disclose it. I can however confirm that the immunogen was an N-terminal partial recombinant human Folliculin protein [Swiss-Prot# Q8NFG4] expressed in E. coli, and the sequence falls within the amino acid range 1-200.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FLCN
Entrez
Uniprot