Folliculin Antibody

Western Blot: Folliculin Antibody [NBP1-44995] - Analysis of FLCN isoforms 1, 2 and 3 on HeLa whole cell lysate

Immunocytochemistry/Immunofluorescence: Folliculin Antibody [NBP1-44995] - Detection of FLCN (Green) in Hela cells using NBP1-44995. Nuclei (Blue) are counterstained using Hoechst 33258

Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. The SCC cells as well as the associated stromal cells depicted strong cytoplasmic-nuclear immunostaining of Folliculin protein. The staining was more intense in the carcinoma cells compared to the tumor stroma [Magnification 10X]

Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human bladder cancer using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. The carcinoma cells as well as the various stromal cells depicted cytoplasmic- nuclear immunostaining for Folliculin protein. The cytoplasmic staining was more intense in the carcinoma cells compared to tumor stroma [Magnification 40X]

Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. Cytoplasmic-nuclear immunostaining of Folliculin protein was observed in the various cells and interestingly, the staining was intense and localized more towards the cell membranes in a bunch of cells having mesenchymal morphology. [Magnification 40X]

Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal skin from human using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. Intense cytoplasmic with moderate nuclear immunostaining of Folliculin protein was observed in the various cells of the epidermal layer [Magnification 40X].

Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal liver of human using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. Strong cytoplasmic-nuclear immunopositivity of Folliculin protein was observed in hepatocytes [Magnification 10X].

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Folliculin Antibody Summary

• Immunogen: N-terminal partial recombinant human Folliculin protein [Swiss-Prot# Q8NFG4] expressed in E. coli.
• Localization: Cytoplasm, Nucleus
• Specificity: This antibody is expected to detect all three isoforms of Folliculin protein ( UniProt # Q8NFG4)
• Predicted Species: Rat (92%), Canine (95%), Primate (99%), Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FLCN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000
• Application Notes: This Folliculin antibody is useful for Western Blot, Immunohistochemistry-Paraffin and Immunocytochemistry/Immunofluorescence applications. In WB, bands are seen at 67 kDa, 37 kDa and 26 kDa reporesenting isoforms 1, 2 and 3 of FLCN respectively.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Human, Mouse. Immunogen sequence similarity to other species: Primate species (99%), Canine (95%), Rat (92%), Bovine (91%), Xenopus (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for Folliculin Antibody

• BHD skin lesion fibrofolliculoma protein
• BHDFLCL
• Birt-Hogg-Dube syndrome protein
• DKFZp547A118
• FLJ45004
• FLJ99377
• folliculin
• MGC17998
• MGC23445

Background

Folliculin (FLCN) is encoded by BHD gene (Birt-Hogg-Dube gene, altered in BHD Syndrome) and is mainly localized in the nucleus, however, has also been observed to co-localize with FLCN-interacting proteins FNIP1 and FNIP2 in the cytoplasm. Highly expressed in several tissues including skin, lung, kidney, heart, testis, stomach etc., FLCN involves in pathogenesis of proliferating epithelial fibrofolliculomas where it is overexpressed, however, its expression is significantly reduced in renal tumor tissues suggesting its tumor suppressor activity in kidneys. FLCN association with FNIP1/FNIP2 mediates its indirect interaction with AMPK subunits (PRKAA1, PRKAB1 and PRKAG1). FLCN has also been suggested to regulate phosphorylation of RPS6KB1 and implicate in energy and/or nutrient sensing through AMPK/mTOR signaling pathways. BHD knock-out mice develop cysts and renal cell tumors similar to those found in BHD patients along with low levels of phosphorylated S6 ribosomal protein. Defects in FLCN are the cause of Birt-Hogg-Dube syndrome (BHD), primary spontaneous pneumothorax (PSP), renal cell carcinoma (RCC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Folliculin Antibody (NBP1-44995)(1)

Publication using NBP1-44995

• Woodford MR, Baker-Williams AJ, Sager RA Et al. The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect Nature structural & molecular biology 2021-08-01 [PMID: 34381247] (ICC/IF, Human)

FAQs for Folliculin Antibody (NBP1-44995). (Showing 1 - 1 of 1 FAQ).

1. Could you please let me know the exact sequence of the immunogen of NBP1-44995?
   • The exact immunogen sequence for our Folliculin antibody with catalogue number NBP1-44995 is proprietary, and I am unable to disclose it. I can however confirm that the immunogen was an N-terminal partial recombinant human Folliculin protein [Swiss-Prot# Q8NFG4] expressed in E. coli, and the sequence falls within the amino acid range 1-200.

Bioinformatics

• Gene Symbol: FLCN
• Entrez:
  • 201163(Human)
  • 216805(Mouse)
• Uniprot:
  • Q8NFG4(Human)
  • Q8QZS3(Mouse)