FMRP Antibody (OTI1C6) - Azide and BSA Free

Western Blot: FMRP Antibody (OTI1C6) - Azide and BSA Free [NBP2-70756] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FMRP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FMRP.

Immunocytochemistry/Immunofluorescence: FMRP Antibody (OTI1C6) - Azide and BSA Free [NBP2-70756] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FMRP.

Immunohistochemistry: FMRP Antibody (OTI1C6) - Azide and BSA Free [NBP2-70756] - Staining of paraffin-embedded Human colon tissue using anti-FMRP mouse monoclonal antibody.

Flow Cytometry: FMRP Antibody (OTI1C6) - Azide and BSA Free [NBP2-70756] - Analysis of Jurkat cells, using anti-FMRP antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: FMRP Antibody (OTI1C6) - Azide and BSA Free [NBP2-70756] - Analysis of extracts (35ug) from 9 different cell lines by using anti-FMRP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: FMRP Antibody (OTI1C6) - Azide and BSA Free [NBP2-70756] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FMRP mouse monoclonal antibody.

Immunohistochemistry: FMRP Antibody (OTI1C6) - Azide and BSA Free [NBP2-70756] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FMRP mouse monoclonal antibody.

Immunohistochemistry: FMRP Antibody (OTI1C6) - Azide and BSA Free [NBP2-70756] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FMRP mouse monoclonal antibody.

Immunohistochemistry: FMRP Antibody (OTI1C6) - Azide and BSA Free [NBP2-70756] - Staining of paraffin-embedded Human pancreas tissue using anti-FMRP mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

FMRP Antibody (OTI1C6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse FMRP Antibody (OTI1C6) - Azide and BSA Free (NBP2-01770) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 36-279 of human FMR1(NP_002015) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FMR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for FMRP Antibody (OTI1C6) - Azide and BSA Free

• FMRPFRAXAPOF
• fragile X mental retardation 1 protein
• fragile X mental retardation 1
• MGC87458
• POF1
• premature ovarian failure 1
• Protein FMR-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FMR1