FMO2 Overexpression Lysate (Native)

Images

 
Western Blot: FMO2 Overexpression Lysate (Adult Normal) [NBL1-10776] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FMO2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FMO2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203237 and based on accession number NM_001460. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens flavin containing monooxygenase 2 (non-functional) (FMO2), mRNA.
Gene
FMO2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
53.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for FMO2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FMO2 Overexpression Lysate (Native)

  • dimethylaniline monooxygenase [N-oxide-forming] 2
  • Dimethylaniline oxidase 2
  • EC 1.14.13.8
  • flavin containing monooxygenase 2 (non-functional)
  • flavin containing monooxygenase 2
  • FLJ40826
  • FMO 1B1
  • FMO 2
  • FMO, pulmonary
  • FMO1B1
  • Pulmonary flavin-containing monooxygenase 2

Background

The flavin-containing monooxygenases are NADPH-dependent enzymes that catalyze the oxidation of many drugs and xenobiotics. In most mammals, there is a flavin-containing monooxygenase that catalyzes the N-oxidation of some primary alkylamines through an N-hydroxylamine intermediate. However, in humans, this enzyme is truncated and is probably rapidly degraded. The protein encoded by this gene represents the truncated form and apparently has no catalytic activity. A functional allele found in African Americans has been reported, but no sequence evidence has been deposited to support the finding. This gene is found in a cluster with the FMO1, FMO3, and FMO4 genes on chromosome 1. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for FMO2 Lysate (NBL1-10776) (0)

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Bioinformatics

Gene Symbol FMO2