FLRT2 Antibody [Unconjugated]

Western Blot shows lysates of NCI‑H460 human large cell lung carcinoma cell line. PVDF membrane was probed with 1 µg/ml of Goat Anti-Human FLRT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2877) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FLRT2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western shows lysates of NCI‑H460 human large cell lung carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for FLRT2 at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FLRT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2877). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

FLRT2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human FLRT2
  Cys36-Ser539
  Accession # O43155
• Specificity: Detects human FLRT2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FLRT2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FLRT2 Antibody [Unconjugated]

• Fibronectin Leucine Rich Transmembrane Protein 2
• Fibronectin-Like Domain-Containing Leucine-Rich Transmembrane Protein 2
• FLRT2
• KIAA0405
• Leucine-Rich Repeat Transmembrane Protein FLRT2

Background

FLRT2 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85 kDa type I transmembrane (TM) human FLRT2 is synthesized as a 660 amino acid (aa) precursor with a 35 aa signal sequence, a 506 aa extracellular domain (ECD), a 21 aa TM segment and a 98 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT2 ECD shares 97%, 96%, 99%, 96% and 95% aa sequence identity with mouse, rat, equine, canine and bovine FLRT2 ECD, respectively. Human FLRT1 and FLRT3 ECDs share approximately 47% aa identity with FLRT2. The fibronectin domain of all three FLRTs can bind to FGF receptors (2). This binding is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization of FLRTs in areas of cell contact and homotypic cell-cell association (4). This may be through direct interactions with other FLRT molecules or, as has been shown for FLRT3, by regulating internalization of adhesion molecules such as cadherins (4, 5). In adulthood, FLRT2 mRNA is most abundant in pancreas, but is also present in skeletal muscle, brain and heart (1). FLRT2 in mouse embryos shows highest expression in a subset of the sclerotome in the brain, the stomach, and posterior to the developing heart (2). This expression is distinct from that of FLRT1 and FLRT3 (2).

1. Lacy, S. E. et al. (1999) Genomics 62:417.
2. Haines, B. P. et al. (2006) Dev. Biol. 297:14.
3. Bottcher, R. T. et al. (2004) Nat. Cell Biol. 6:38.
4. Karaulanov, E. E. et al. (2006) EMBO Rep. 7:283.
5. Ogata, S. et al. (2007) Genes Dev. 21:1817.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FLRT2 Antibody (AF2877)(4)

Publications using AF2877

• Ando T, Tai-Nagara I, Sugiura Y et al. Tumor-specific inter-endothelial adhesion mediated by FLRT2 facilitates cancer aggressiveness The Journal of clinical investigation 2/1/2022 [PMID: 35104247]
• H Blockus, SV Rolotti, M Szoboszlay, E Peze-Heids, T Ming, A Schroeder, N Apostolo, KM Vennekens, PS Katsamba, F Bahna, S Mannepalli, G Ahlsen, B Honig, L Shapiro, J de Wit, A Losonczy, F Polleux Synaptogenic activity of the axon guidance molecule Robo2 underlies hippocampal circuit function Cell Reports, 2021-10-19;37(3):109828. 2021-10-19 [PMID: 34686348] (IHC, Mouse)
• Chiara Camillo, Nicola Facchinello, Giulia Villari, Giulia Mana, Noemi Gioelli, Chiara Sandri et al. LPHN2 inhibits vascular permeability by differential control of endothelial cell adhesion Journal of Cell Biology 2021-11-01 [PMID: 34581723] (Western Blot, Human)
• I Tai-Nagara, Y Yoshikawa, N Numata, T Ando, K Okabe, Y Sugiura, M Ieda, N Takakura, O Nakagawa, B Zhou, K Okabayashi, M Suematsu, Y Kitagawa, M Bastmeyer, K Sato, R Klein, S Navankasat, DY Li, S Yamagishi, Y Kubota Placental labyrinth formation in mice requires endothelial FLRT2-UNC5B signaling Development, 2017-06-02;0(0):. 2017-06-02 [PMID: 28576770] (IHC, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: FLRT2
• Uniprot:
  • O43155()