FLRT3 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

FLRT3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human FLRT3
  Lys29-Pro528
  Accession # Q9NZU0
• Specificity: Detects human FLRT3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human FLRT1 and rhFLRT2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FLRT3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FLRT3 Antibody [Unconjugated]

• fibronectin leucine rich transmembrane protein 3
• Fibronectin-like domain-containing leucine-rich transmembrane protein 3
• FLRT3
• HH21
• KIAA1469
• leucine-rich repeat transmembrane protein FLRT3

Background

FLRT3 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85-95 kDa type I transmembrane (TM) human FLRT3 is synthesized as a 649 amino acid (aa) precursor with a 28 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa TM segment and a 100 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT3 ECD shares 96%, 96%, 97%, 97%, 98%, and 81% aa sequence identity with mouse, rat, canine, bovine, equine, and Xenopus FLRT3 ECD, respectively, and 61% and 48% aa identity to human FLRT2 and FLRT3 ECDs, respectively. The fibronectin domain is responsible for binding to FGF receptors, and is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization in areas of cell contact and homotypic cell-cell association (4). This may be through direct interaction with other FLRT molecules, or alternatively, by regulating internalization of adhesion molecules such as cadherins (4, 5). Developmentally, FLRT3 is located in somitic regions on dermatomyotomal muscle precursors and myotomal cells before their migration to the myotome and syndetome, respectively (2). FLRT3 is also expressed at the midbrain/hindbrain boundary and in the apical ectodermal ridge where it may influence FGF signaling (2). Genetic deletion in mouse embryos results in defective headfold fusion and endoderm migration (6). Postnatally, FLRT3 mRNA is widely expressed (1). It is upregulated and promotes neurite outgrowth following experimental peripheral nerve injury in rats (7, 8).

 

1. Lacy, S.E. et al. (1999) Genomics 62:417.
2. Haines, B.P. et al. (2006) Dev. Biol. 297:14.
3. Bottcher, R.T. et al. (2004) Nat. Cell Biol. 6:38.
4. Karaulanov, E.E. et al. (2006) EMBO Rep. 7:283.
5. Ogata, S. et al. (2007) Genes Dev. 21:1817.
6. Maretto, S. et al. (2008) Dev. Biol. 318:184.
7. Tsuji, L. et al. (2004) Biochem. Biophys. Res. Commun. 313:1086.
8. Robinson, M. et al. (2004) Mol. Cell. Neurosci. 27:202.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FLRT3 Antibody (AF2795)(7)

Publications using AF2795

• Despin-Guitard, E;Rosa, VS;Plunder, S;Mathiah, N;Van Schoor, K;Nehme, E;Merino-Aceituno, S;Egea, J;Shahbazi, MN;Theveneau, E;Migeotte, I; Non-apical mitoses contribute to cell delamination during mouse gastrulation Nature communications 2024-08-28 [PMID: 39198421] (Immunohistochemistry, Mouse)
• Partiot, E;Hirschler, A;Colomb, S;Lutz, W;Claeys, T;Delalande, F;Deffieu, MS;Bare, Y;Roels, JRE;Gorda, B;Bons, J;Callon, D;Andreoletti, L;Labrousse, M;Jacobs, FMJ;Rigau, V;Charlot, B;Martens, L;Carapito, C;Ganesh, G;Gaudin, R; Brain exposure to SARS-CoV-2 virions perturbs synaptic homeostasis Nature microbiology 2024-03-28 [PMID: 38548923] (Immunocytochemistry, Human)
• Xiaofen Li, Qirui Zhao, Xiaojie Yu, Wenhan Cao, Yingyi Zhang, Wanying Feng et al. Apicosome: Newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cells iScience 2023-04-21 [PMID: 37123250]
• Shanshan Li, Huoqing Luo, Ronghui Lou, Cuiping Tian, Chen Miao, Lisha Xia et al. Multiregional profiling of the brain transmembrane proteome uncovers novel regulators of depression Science Advances 2021-07-23 [PMID: 34290087]
• Del Toro D, Carrasquero-Ordaz MA, Chu A et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons Cell 1/23/2020 [PMID: 31928845]
• M Yamada, Y Fujita, Y Hayano, H Hayakawa, K Baba, H Mochizuki, T Yamashita Increased expression of fibronectin leucine-rich transmembrane protein 3 in the dorsal root ganglion induces neuropathic pain in rats J. Neurosci., 2019-07-25;0(0):. 2019-07-25 [PMID: 31346030] (IHC, In Vivo, Western Blot, Rat)
• Egea J, Erlacher C, Montanez E, Burtscher I, Yamagishi S, Hess M, Hampel F, Sanchez R, Rodriguez-Manzaneque MT, Bosl MR, Fassler R, Lickert H, Klein R Genetic ablation of FLRT3 reveals a novel morphogenetic function for the anterior visceral endoderm in suppressing mesoderm differentiation. Genes Dev., 2008-12-01;22(23):3349-62. 2008-12-01 [PMID: 19056886] (IHC, Mouse)

Bioinformatics

• Gene Symbol: FLRT3
• Uniprot:
  • Q9NZU0()