FLIP Antibody (896537) [Alexa Fluor® 750]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 896537
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

FLIP Antibody (896537) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human FLIP
  Met1-Asn200
  Accession # O15519
• Specificity: Detects human FLIP in direct ELISAs and human, mouse, and rat FLIP in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FLIP Antibody (896537) [Alexa Fluor® 750]

• CASHFADD-like antiapoptotic molecule 1
• CASP8 and FADD-like apoptosis regulator
• CASP8AP1
• Caspase homolog
• Caspase-eight-related protein
• Caspase-like apoptosis regulatory protein
• caspase-related inducer of apoptosis
• CASPER
• CasperFLAME1
• Cellular FLICE-like inhibitory protein
• CFLAR
• c-FLIPR
• c-FLIPS
• CLARPMACH-related inducer of toxicity
• FADD-like apoptotic molecule
• FLAME
• FLAME-1
• FLIP
• I-FLICE
• I-FLICEMRITc-FLIPFLIPc-FLIPL
• Inhibitor of FLICE
• usurpin beta
• Usurpin

Background

FLIP (Flice-Like Inhibitory Protein), also known as CFLAR (CASP8 and FADD-Like Apoptosis Regulator), I-FLICE, CASPER, and FLAME-1, is an apoptosis inhibitory protein with architecture similar to that of Caspases-8 and -10. Both of the major FLIP isoforms, the 55 kDa Long (L) and the 27 kDa Short (S), contain two death effector domains (DED).  FLIP(L) has a C-terminal Caspase-like protease domain which lacks both a catalytic active site and residues that form a substrate-binding pocket. FLIP(S) and FLIP(L) interact with the adaptor molecule FADD, and potently inhibit apoptosis initiated by Fas Ligand/TNF signaling pathways.  Over amino acids 1-200, human FLIP shares 75% sequence identity with mouse and rat FLIP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.