FLIP Antibody (896537) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 896537
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

FLIP Antibody (896537) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human FLIP
  Met1-Asn200
  Accession # O15519
• Specificity: Detects human FLIP in direct ELISAs and human, mouse, and rat FLIP in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CFLAR
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for FLIP Antibody (896537) [CoraFluor™ 1]

• CASHFADD-like antiapoptotic molecule 1
• CASP8 and FADD-like apoptosis regulator
• CASP8AP1
• Caspase homolog
• Caspase-eight-related protein
• Caspase-like apoptosis regulatory protein
• caspase-related inducer of apoptosis
• CASPER
• CasperFLAME1
• Cellular FLICE-like inhibitory protein
• CFLAR
• c-FLIPR
• c-FLIPS
• CLARPMACH-related inducer of toxicity
• FADD-like apoptotic molecule
• FLAME
• FLAME-1
• FLIP
• I-FLICE
• I-FLICEMRITc-FLIPFLIPc-FLIPL
• Inhibitor of FLICE
• usurpin beta
• Usurpin

Background

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CFLAR