Fibulin 5/DANCE Antibody

Images

 
Western Blot: Fibulin 5/DANCE Antibody [NB100-53783] - (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Fibulin 5/DANCE Antibody Summary

Immunogen
Peptide with sequence C-RPIKGPREIQLDLE corresponding to internal region (near C-Terminus) according to NP_006320.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FBLN5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 50 kDa band observed in human colon, heart and ovary lysates (calculated MW of 50.2 kDa band according to NP_006320.2). An additional band of unknown identity was also consistently observed at 23 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Positive Control
Ovary Lysate (NB820-59243)
Fibulin 5/DANCE Lysate (NBL1-10609)
Publications
Read Publication using NB100-53783.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fibulin 5/DANCE Antibody

  • ARMD3fibulin-5
  • DANCE
  • DANCEFIBL-5
  • Developmental arteries and neural crest EGF-like protein
  • EVEC
  • FBLN5
  • Fibulin 5
  • UP50
  • UP50FLJ90059
  • Urine p50 protein

Background

The protein encoded by this gene is a secreted, extracellular matrix protein containing an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. It is prominently expressed in developing arteries but less so in adult vessels. However, its expression is reinduced in balloon-injured vessels and atherosclerotic lesions, notably in intimal vascular smooth muscle cells and endothelial cells. Therefore, the protein encoded by this gene may play a role in vascular development and remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fibulin 5/DANCE Antibody (NB100-53783)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FBLN5
Entrez
Uniprot