Fibulin 5/DANCE Antibody (293904) [Alexa Fluor™ Plus 488]

• Reactivity: Hu
• Clone: 293904
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 488

Fibulin 5/DANCE Antibody (293904) [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects human Fibulin 5/DANCE in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibulin 5/DANCE Antibody (293904) [Alexa Fluor™ Plus 488]

• ARMD3fibulin-5
• DANCE
• DANCEFIBL-5
• Developmental arteries and neural crest EGF-like protein
• EVEC
• FBLN5
• Fibulin 5
• UP50
• UP50FLJ90059
• Urine p50 protein

Background

Fibulin 5, also known as DANCE and EVEC, is a secreted 55 kDa matricellular glycoprotein that plays an important role in elastic fiber network assembly and angiogenesis (1). Mature human Fibulin 5 contains an N-terminal EGF-like domain with an RGD motif, a 44 amino acid (aa) spacer region, five more tandem EGF-like domains, and a 115 aa Fibulin-like C-terminal region (2, 3). Mature human Fibulin 5 shares 95% aa sequence identity with mouse and rat Fibulin 5. Fibulin 5 is expressed by smooth muscle cells and endothelial cells of the developing vasculature as well as by migrating neural crest cells and lung interstitial fibroblasts (2‑4). It is down‑regulated in the adult vasculature but is re‑expressed at aortic branching points, in the uterus, and at sites of mechanical or atherosclerotic injury (2, 3, 5). The RGD motif of Fibulin 5 binds to several cell surface Integrins including alpha V beta 3, alpha V beta 5, alpha 9 beta 1, alpha 4 beta 1, and alpha 5 beta 1 (2, 6, 7). The calcium-dependent binding of Fibulin 5 to elastic fibers serves to anchor cells to the extracellular matrix (8). Fibulin 5 promotes elastic fiber assembly and maturation by organizing Tropoelastin, LTBP-2, and the crosslinking lysyl oxidase-like enzymes LOXL1, 2, and 4 along Fibrillin microfibrils (6, 9‑11). In aged mice with decreased tissue elasticity, proteolytic removal of the N-terminal EGF-like domain prevents Fibulin 5 from interacting with Fibrillin-1 microfibrils (10). Fibulin 5 functions as an angiogenesis inhibitor by inhibiting vascular smooth muscle proliferation and migration and by limiting vascular sprouting (5, 12). Fibulin 5 is down‑regulated in many malignancies (15). When experimentally re‑expressed, however, Fibulin 5 enhances tumor cell invasiveness and cooperates with TGF-beta in initiating and promoting epithelial-mesenchymal transition (EMT) (13, 15). Defects in Fibulin 5 expression or function can result in a loss of connective tissue integrity, cardiac elasticity, and ability to remodel the vasculature after injury (8, 5, 14).

1. Yanagisawa, H. et al. (2009) J. Cell. Commun. Signal. 3:337.
2. Nakamura, T. et al. (1999) J. Biol. Chem. 274:22476.
3. Kowal, R.C. et al. (1999) Circ. Res. 84:1166.
4. Kuang, P.-P. et al. (2003) Am. J. Physiol. Lung Cell. Mol. Physiol. 285:L1147.
5. Spencer, J.A. et al. (2005) Proc. Natl. Acad. Sci. 102:2946.
6. Nakamura, T. et al. (2002) Nature 415:171.
7. Lomas, A.C. et al. (2007) Biochem. J. 405:417.
8. Yanagisawa, H. et al. (2002) Nature 415:168.
9. Wachi, H. et al. (2008) J. Biochem. 143:633.
10. Hirai, M. et al. (2007) J. Cell Biol. 176:1061.
11. Hirai, M. et al. (2007) EMBO J. 26:3283.
12. Sullivan, K.M. et al. (2007) Lab. Invest. 87:818.
13. Lee, Y.-H. et al. (2008) Carcinogenesis 29:2243.
14. Loeys, B. et al. (2002) Hum. Mol. Genet. 11:2113.
15. Schiemann, W.P. et al. (2002) J. Biol. Chem. 277:27367.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.