Fibulin-3/EFEMP1 Antibody

Western blot shows lysates of HUVEC human umbilical vein endothelial cells and human muscle tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Fibulin 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6235) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibulin 3 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Fibulin-3/EFEMP1 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Fibulin 3
  Gln19-Phe493
  Accession # Q12805
• Specificity: Detects human Fibulin 3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: EFEMP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibulin-3/EFEMP1 Antibody

• DHRD
• EFEMP1
• EGF containing fibulin-like extracellular matrix protein 1
• EGF-containing fibulin-like extracellular matrix protein 1
• Extracellular protein S1-5
• FBLN3
• FBLN3DRAD
• FBNLFLJ35535
• FIBL-3
• Fibrillin-like protein
• fibrillin-like
• Fibulin 3
• fibulin-3
• MGC111353
• MLVT
• MTLV
• S1-5

Background

Fibulin 3 (also S1-5 and EFEMP1) is a secreted, monomeric 55‑57 kDa member of the fibulin family of proteins. In mouse, Fibulin 3 appears in an O-glycosylated 63 and 80 kDa form. Given the high amino acid (aa) conservation between mouse and human, human Fibulin 3 may also demonstrate like isoforms. Fibulin 3 is expressed by fibroblasts, endothelial cells and prechondrocytes, and appears to provide both stability to ECM (elastin and basement membrane), and to regulate cell maturation/ differentiation. Fibulin 3 is reported to activate the EGF receptor, an effect that may influence multiple cell types under multiple scenarios. Mature human Fibulin 3 is 476 aa in length. It contains six EGF-like domains (aa 26‑378), at least one of which binds to the EGF receptor and induces EGFR phosphorylation. There is at least one alternative start site at Met107 that generates a 43 kDa short form. Full-length human Fibulin 3 shares 95% aa identity with mouse Fibulin 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibulin-3/EFEMP1 Antibody (AF6235)(1)

Publication using AF6235

• Akihiko Hasegawa, Tomo Yonezawa, Noboru Taniguchi, Koji Otabe, Yukio Akasaki, Tetsuya Matsukawa et al. Role of Fibulin 3 in Aging-Related Joint Changes and Osteoarthritis Pathogenesis in Human and Mouse Knee Cartilage Arthritis & Rheumatology 2017-03-01 [PMID: 27780308]

Bioinformatics

• Gene Symbol: EFEMP1
• Uniprot:
  • Q12805(Human)