Fibronectin Antibody


Biological Strategies: Western Blot: Fibronectin Antibody [NBP1-31369] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with more
Immunocytochemistry/ Immunofluorescence: Fibronectin/Anastellin Antibody [NBP1-31369] - Analysis of HeLa, using NBP1-31369 at 1:200 dilution.
Immunohistochemistry-Paraffin: Fibronectin/Anastellin Antibody [NBP1-31369] - Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term dilution: 1:500.
Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Whole cell extracts (30 ug) was separated by 5 % SDS-PAGE, and blotted with Fibronectin antibody [N1N2], N-term diluted by 1:1000
Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] diluted at 1:1000.
Immunoprecipitation: Fibronectin/Anastellin Antibody [NBP1-31369] - N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of more
ELISA: Fibronectin Antibody [NBP1-31369] - Indirect ELISA analysis was performed by coating plate with 100 uL of recombinant Fibronectin protein at concentration of 10 ug/mL. The coated protein is detected with more
ELISA: Fibronectin Antibody [NBP1-31369] - Competitive ELISA detection of human fibronectin in plasma. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 ug/ml. Human more

Product Details

Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Validated by:

Biological Strategies


Order Details

Fibronectin Antibody Summary

Recombinant protein encompassing a sequence within the N-terminus region of human Fibronectin. The exact sequence is proprietary.
Secreted, extracellular space, extracellular matrix
Mesenchymal Cells Marker
Predicted Species
Bovine (92%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Application Notes
IHC-Fr (Frozen sections) Assay dependent
Theoretical MW
263 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken 88%.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 1% BSA, 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified

Alternate Names for Fibronectin Antibody

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF


Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fibronectin Antibody (NBP1-31369). (Showing 1 - 1 of 1 FAQ).

  1. For NBP1-31369 the Fibronectin antibody, do I need to add any other buffer solution before aliquotting it?
    • For the Fibronectin antibody (NBP1-31369) you do not need to add anything else to the buffer to store it in your aliquots. It already has glycerol, which will keep it stable at lower temperatures and the main thing is to avoid repeated freeze thaw cycles that might cause your antibody to lose binding affinity.

Secondary Antibodies


Isotype Controls

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Gene Symbol FN1