Fibronectin Antibody (DH1)

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Product Details

Summary
Reactivity Hu, Rt, Ch, GP, RbSpecies Glossary
Applications WB, IHC, IHC-P
Clone
DH1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Fibronectin Antibody (DH1) Summary

Immunogen
The antibody reacts with Fibronectin.
Marker
Mesenchymal Cells Marker
Specificity
The antibody is specific to extradomain A (EDA) sequence of a cellular fibronectin and recognizes thus only the cellular fibronectin. Mab DHI has been shown to specifically block chondrocyte condensation in chicken embryos (5). The antibody cross reacts with human, chicken, rat, rabbit and guinea pig. Fibronectins are diverse high molecular weight cell adhesion glycoproteins found in basement membranes, in the interstitial connective tissue matrix, and in soluble in plasma and other body fluids. In adult tissues most fibronectin is produced by hepatocytes (pFn) that differ from locally produced cellular fibronectins. A monoclonal antibody (BF12) has been produced to fibronectin that reacts with both the cellular and plasma forms of the protein and recognizes the cell-binding region in fibronectin. The monoclonal antibody DH1 recognizes only cellular form of Fibronectin. DH1 is specific to the extra domain A (EDA) of the molecule. cFns are found in some basement membranes during development and only in distinct tissues in adult, for instance in smooth muscle and alveolar walls of airways. cFns are abundant in the plasma of carcinoma patients and in the stroma of various carcinomas. Monoclonal antibody (Mab) to cFn is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Purified fibronectin from A8387 fibrosarcoma cells was used as an immunogen (21).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FN1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Immunohistochemical stainings on paraffin sections, western blotting and other immunoassays for cellular and tumor biology.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
100 mg immunoglobulin with 1 ml PBS and 1.0% (w/v) BSA
Preservative
0.1% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for Fibronectin Antibody (DH1)

  • Fibronectin
  • FN1

Background

Integrin beta1 also known as integrin VLA-4 beta chain, fibronectin receptor beta chain, and CD29) is a 130 kD integrin beta chain containing VWFA-like domains. Five isoforms have been reported (beta1A-1D, 1C-2). This Type I membrane protein is a subunit of the heterodimeric receptor for laminin, fibronectin, collagen, fibrinogen, VCAM1, cytotactin, osteopontin, epiligrin, thrombospondin, and vitronectin. Signal transduction through the integrins affects embryogenesis, hemostasis, tissue repair, immune response, and metastatic spread of tumor cells. Integrin beta1 can be upregulated by LPS with soluble CD14 and TNF-alpha; downregulation of integrin beta1 by alpha-melanocyte stimulating hormone and progesterone. Integrin beta1 is modified by phosphorylation and glycosylation. Integrin beta1 partners with integrin alpha subunits alphav, and alpha1 through alpha1.1. The Poly6004 antibody recognizes the N-terminal region of human integrin beta1 and has been shown to be useful for Western blotting. Partners with integrin alpha subunits alphav, alpha1 through alpha11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Fibronectin/Anastellin Antibody (NBP1-51723). (Showing 1 - 1 of 1 FAQ).

  1. What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.
    • I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NBP1-51723, the dilution range is 1:500-1:1000.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Fibronectin/Anastellin Antibody (NBP1-51723)

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Blogs on Fibronectin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Fibronectin: Organizing Cell Activity across the ECM
Fibronectin is a glycoprotein found in the extracellular matrix (ECM) that binds to integrins and other components of the ECM such as collagen and fibrin. Under normal physiological conditions, fibronectin is an important factor in cell adhesion, grow...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Bioinformatics

Gene Symbol FN1
Entrez