Fibronectin Antibody (A32) - BSA Free

• Reactivity: Hu, Bv
• Applications: WB, ELISA, IHC, IP
• Clone: A32
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Fibronectin Antibody (A32) - BSA Free Summary

• Immunogen: Bovine corneal endothelial cells
• Epitope: Epitope is located in the 40kD Hep II heparin-binding domain, but differs from that of Clone A32
• Marker: Mesenchymal Cells Marker
• Specificity: NBP2-23525 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: NBP2-23525 can be used in ELISA. It can be used to probe fibronectin conformation and to quantitate plasma fibronectin in a sandwich ELISA with antibody NBP1-05056. (1, 2, 3, 4) WB: In Western blotting dilution guideline of 1/100 has proved successful. (1) IHC: NBP2-23525 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. IP: NBP2-23525 can be used in immunoprecipitation.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Fibronectin Antibody (A32) - BSA Free

• CIG
• ED-B
• fibronectin 1
• Fibronectin
• FINC
• FN
• FN1
• FNZ
• GFND
• GFND2
• LETS
• MSF
• SMDCF

Background

Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FN1