Fibronectin Antibody (2F4)

Images

 
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody (2F4) [NBP2-22113] - ICC/IF analysis of cultured HepG2 cells using Fibronectin antibody (clone 2F4) at 1:100 dilution. The cells were fixed in formalin and ...read more
Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Western blot analysis of HeLa (1) and HepG2 (2) whole cell lysates using Fibronectin antibody (2F4) at 2 ug/ml.
Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using Fibronectin mouse mAb with DAB staining.
Flow Cytometry: Fibronectin Antibody (2F4) [NBP2-22113] - Flow cytometric analysis of HeLa cells using Fibronectin mouse mAb (green) and negative control (red).
Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Western blot analysis using Fibronectin mAb against human Fibronectin (AA: 1965-2176) recombinant protein. (Expected MW is 49.6 kDa)
Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Western blot analysis using Fibronectin mAb against HEK293 (1) and Fibronectin (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Fibronectin mouse mAb with DAB staining.
ELISA: Fibronectin Antibody (2F4) [NBP2-22113] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
2F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Fibronectin Antibody (2F4) Summary

Immunogen
Purified recombinant fragment of human Fibronectin expressed in E. coli. [UniProt# P02751]
Marker
Mesenchymal Cells Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FN1
Purity
Ammonium sulfate precipitation
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200-11:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This Fibronectin (2F4) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
262.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Fibronectin Antibody (2F4)

  • Fibronectin
  • FN1

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fibronectin Antibody (NBP2-22113) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Fibronectin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Fibronectin: Organizing Cell Activity across the ECM
Fibronectin is a glycoprotein found in the extracellular matrix (ECM) that binds to integrins and other components of the ECM such as collagen and fibrin. Under normal physiological conditions, fibronectin is an important factor in cell adhesion, grow...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Bioinformatics

Gene Symbol FN1
Entrez
Uniprot