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Fibronectin Antibody (2F4)

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Immunocytochemistry/Immunofluorescence: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of cultured HepG2 cells using Fibronectin antibody (clone 2F4) at 1:100 dilution. The cells were fixed in formalin and permeabilized using PBS with 0.1% Triton X-100 before incubating them with Fibronectin antibody or the mouse IgG1 isotype control antibody. DyLight 488 conjugated goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody was used at 1: 200 dilution to detect the antigen-primary antibody complexes and the cells were counterstained with DAPI for labeling the nuclei. This Fibronectin antibody generated the expected punctate cytoplasmic-membranous staining and the signal was localized more to the inter-cellular spaces in clustered cells.
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of cultured HepG2 cells using Fibronectin antibody (clone 2F4) at 1:100 dilution. The cells were fixed in formalin and ...read more
Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of HeLa (1) and HepG2 (2) whole cell lysates using Fibronectin antibody (2F4) at 2 ug/mL.
Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of paraffin-embedded stomach cancer tissues using Fibronectin mouse mAb with DAB staining.
Flow Cytometry: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of HeLa cells using Fibronectin mouse mAb (green) and negative control (red).
Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis using Fibronectin mAb against human Fibronectin (AA: 1965-2176) recombinant protein. Expected MW is 49.6 kDa.
Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis using Fibronectin mAb against HEK293 (1) and Fibronectin (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of paraffin-embedded lung cancer tissues using Fibronectin mouse mAb with DAB staining.
ELISA: Fibronectin Antibody (2F4) [NBP2-22113] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
2F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22113AF350
Alexa Fluor 405
NBP2-22113AF405
Alexa Fluor 488
NBP2-22113AF488
Alexa Fluor 532
NBP2-22113AF532
Alexa Fluor 594
NBP2-22113AF594
Alexa Fluor 647
NBP2-22113AF647
Alexa Fluor 700
NBP2-22113AF700
Alexa Fluor 750
NBP2-22113AF750
Allophycocyanin
NBP2-22113APC
Allophycocyanin/Cy7
NBP2-22113APCCY7
Biotin
NBP2-22113B
DyLight 350
NBP2-22113UV
DyLight 405
NBP2-22113V
DyLight 488
NBP2-22113G
DyLight 550
NBP2-22113R
DyLight 594
NBP2-22113DL594
DyLight 650
NBP2-22113C
DyLight 680
NBP2-22113FR
DyLight 755
NBP2-22113IR
FITC
NBP2-22113F
HRP
NBP2-22113H
Janelia Fluor 549
NBP2-22113JF549
Janelia Fluor 646
NBP2-22113JF646
PE
NBP2-22113PE
PE/Atto594
NBP2-22113PEATT594
PE/Cy5.5
NBP2-22113PECY55
PE/Cy7
NBP2-22113PECY7
PerCP
NBP2-22113PCP
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Fibronectin Antibody (2F4) Summary

Immunogen
Purified recombinant fragment of human Fibronectin expressed in E. coli. [UniProt# P02751]
Marker
Mesenchymal Cells Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FN1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200 - 11:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
262.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Fibronectin Knockout HeLa Cell Lysate
Publications
Read Publication using NBP2-22113.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Fibronectin Antibody (2F4)

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF
  • SMDCF

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fibronectin Antibody (NBP2-22113)(1)


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Product General Protocols

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WB Video Protocol
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Control Lysate(s)

Fibronectin Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22113AF350
Alexa Fluor 405 NBP2-22113AF405
Alexa Fluor 488 NBP2-22113AF488
Alexa Fluor 532 NBP2-22113AF532
Alexa Fluor 594 NBP2-22113AF594
Alexa Fluor 647 NBP2-22113AF647
Alexa Fluor 700 NBP2-22113AF700
Alexa Fluor 750 NBP2-22113AF750
Allophycocyanin NBP2-22113APC
Allophycocyanin/Cy7 NBP2-22113APCCY7
Biotin NBP2-22113B
DyLight 350 NBP2-22113UV
DyLight 405 NBP2-22113V
DyLight 488 NBP2-22113G
DyLight 550 NBP2-22113R
DyLight 594 NBP2-22113DL594
DyLight 650 NBP2-22113C
DyLight 680 NBP2-22113FR
DyLight 755 NBP2-22113IR
FITC NBP2-22113F
HRP NBP2-22113H
Janelia Fluor 549 NBP2-22113JF549
Janelia Fluor 646 NBP2-22113JF646
PE NBP2-22113PE
PE/Atto594 NBP2-22113PEATT594
PE/Cy5.5 NBP2-22113PECY55
PE/Cy7 NBP2-22113PECY7
PerCP NBP2-22113PCP
Learn about Custom Antibody Labeling

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By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric...  Read full blog post.
Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
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Bioinformatics

Gene Symbol FN1
Entrez
Uniprot