Fibronectin Antibody (2F4) - BSA Free

Immunocytochemistry/Immunofluorescence: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of cultured HepG2 cells using Fibronectin antibody (clone 2F4) at 1:100 dilution. The cells were fixed in formalin and permeabilized using PBS with 0.1% Triton X-100 before incubating them with Fibronectin antibody or the mouse IgG1 isotype control antibody. DyLight 488 conjugated goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody was used at 1: 200 dilution to detect the antigen-primary antibody complexes and the cells were counterstained with DAPI for labeling the nuclei. This Fibronectin antibody generated the expected punctate cytoplasmic-membranous staining and the signal was localized more to the inter-cellular spaces in clustered cells.

Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of HeLa (1) and HepG2 (2) whole cell lysates using Fibronectin antibody (2F4) at 2 ug/mL.

Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of paraffin-embedded stomach cancer tissues using Fibronectin mouse mAb with DAB staining.

Flow Cytometry: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of HeLa cells using Fibronectin mouse mAb (green) and negative control (red).

Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis using Fibronectin mAb against human Fibronectin (AA: 1965-2176) recombinant protein. Expected MW is 49.6 kDa.

Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis using Fibronectin mAb against HEK293 (1) and Fibronectin (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of paraffin-embedded lung cancer tissues using Fibronectin mouse mAb with DAB staining.

ELISA: Fibronectin Antibody (2F4) [NBP2-22113] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
• Clone: 2F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Fibronectin Antibody (2F4) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Fibronectin expressed in E. coli. [UniProt# P02751]
• Marker: Mesenchymal Cells Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FN1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200 - 11:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:1000
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 262.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Fibronectin Antibody (2F4) - BSA Free

• CIG
• ED-B
• fibronectin 1
• Fibronectin
• FINC
• FN
• FN1
• FNZ
• GFND
• GFND2
• LETS
• MSF
• SMDCF

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibronectin Antibody (NBP2-22113)(4)

Publications using NBP2-22113

• Radtke AJ, Chu CJ, Yaniv Z Et al. IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues Nat Protoc 2022-01-13 [PMID: 35022622] (IBEX, Human)
  
  Details:
  Citation using the Alexa Fluor 532 version of this antibody.
• Cho HJ, Kim HJ, Lee K et al. Bioengineered Multicellular Liver Microtissues for Modeling Advanced Hepatic Fibrosis Driven Through Non-Alcoholic Fatty Liver Disease Small (Weinheim an der Bergstrasse, Germany) 2021-03-09 [PMID: 33690979]
• Ito Y, Nakamura S et al. Turbulence Activates Platelet Biogenesis to Enable Clinical Scale Ex Vivo Production. Cell 2018-07-26 [PMID: 30017246] (FLOW, Human)
• Hodeib AA, Hassan GFR, Ragab MNM, Hasby EA. Clinical and immunohistochemical comparative study of the efficacy of carboxytherapy vs platelet-rich plasma in treatment of stretch marks. J Cosmet Dermatol 2018-01-07 [PMID: 29316152]

Bioinformatics

• Gene Symbol: FN1
• Entrez:
  • 2335(Human)
• Uniprot:
  • P02751()