FHL2 Antibody

Western Blot: FHL2 Antibody [NBP1-31262] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: FHL2 Antibody [NBP1-31262] - Analysis of HeLa, using FHL2 antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-31262] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Product Discontinued

FHL2 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FHL2. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FHL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for FHL2 Antibody

• AAG11
• down-regulated in rhabdomyosarcoma LIM protein
• DRAL
• DRALAAG11
• FHL2
• FHL-2
• four and a half LIM domains 2
• four and a half LIM domains protein 2
• four and a half LIM-domain protein 2
• LIM domain protein DRAL
• Skeletal muscle LIM-protein 3
• SLIM3
• SLIM-3
• SLIM3aging-associated gene 11

Background

LIM proteins contain a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for FHL2 Antibody (NBP1-31262)(1)

Publication using NBP1-31262

• Nagarajan N, Oka SI, Nah J et al. Thioredoxin 1 promotes autophagy through transnitrosylation of Atg7 during myocardial ischemia The Journal of clinical investigation 2022-12-08 [PMID: 36480290] (WB, Human)

Review for FHL2 Antibody (NBP1-31262) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer Simple Western Rat 10/04/2022

Summary

• Application: Simple Western
• Sample Tested: primary neonatal ventricular cardiomyocytes
• Species: Rat
• Lot: 39715
• Comments: ***Novus Innovators Program - new species or application used on a primary antibody.***

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: FHL2